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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01194
  • pan locus tag?: SAUPAN003511000
  • symbol: SAOUHSC_01194
  • pan gene symbol?: recG
  • synonym:
  • product: ATP-dependent DNA helicase RecG

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01194
  • symbol: SAOUHSC_01194
  • product: ATP-dependent DNA helicase RecG
  • replicon: chromosome
  • strand: +
  • coordinates: 1145830..1147890
  • length: 2061
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    TTGGCTAAAGTAAACTTAATAGAAAGTCCATATTCTCTTTTACAATTAAAAGGTATAGGT
    CCTAAGAAAATAGAAGTATTGCAACAACTAAATATTCATACAGTGGAAGATCTTGTTCTT
    TATTTGCCAACTAGATATGAAGATAATACAGTGATTGATTTGAATCAAGCAGAAGATCAA
    TCTAACGTTACGATAGAAGGACAAGTATATACAGCTCCAGTAGTTGCATTTTTTGGAAGA
    AATAAATCAAAATTAACCGTTCATTTAATGGTAAATAATATTGCTGTCAAATGTATTTTT
    TTCAATCAACCGTATTTAAAAAAGAAAATCGAATTAAATCAAACTATAACTGTTAAAGGT
    AAGTGGAATAGGGTTAAACAGGAAATTACTGGTAATAGGGTTTTCTTTAATTCACAAGGG
    ACACAAACTCAAGAAAACGCAGATGTTCAATTAGAACCAGTCTATCGTATTAAGGAAGGT
    ATTAAACAAAAGCAAATACGAGACCAAATTAGACAAGCGTTAAATGATGTGACAATTCAT
    GAATGGTTAACTGATGAACTAAGAGAAAAATATAAATTAGAGACCTTGGACTTTACTTTG
    AACACATTACATCATCCTAAAAGTAAAGAGGATTTATTACGTGCTCGTAGAACCTATGCA
    TTTACTGAACTGTTTTTATTCGAATTACGTATGCAATGGCTAAATAGATTAGAAAAGTCA
    TCTGACGAAGCAATTGAAATTGATTATGACATAGACCAAGTTAAATCATTTATTGATCGT
    TTACCTTTTGAACTAACTGAAGCACAGAAATCCAGTGTTAATGAAATTTTTAGAGATTTA
    AAAGCACCAATACGTATGCATCGATTACTTCAAGGTGATGTAGGTTCAGGAAAAACAGTA
    GTTGCTGCAATTTGTATGTATGCGTTAAAAACTGCTGGTTATCAATCAGCATTGATGGTA
    CCAACTGAAATTTTAGCAGAGCAACATGCTGAAAGTTTAATGGCTTTATTTGGAGATTCT
    ATGAACGTTGCATTGTTAACTGGGTCAGTAAAAGGTAAGAAACGAAAGATACTTTTAGAA
    CAACTTGAAAATGGTACGATTGATTGTTTAATTGGAACCCATGCTTTGATTCAAGATGAT
    GTGATTTTCCATAATGTTGGTTTAGTAATTACAGATGAACAACATCGATTTGGTGTGAAT
    CAACGCCAGCTTTTAAGAGAAAAAGGTGCAATGACGAATGTGTTATTTATGACAGCAACG
    CCGATACCAAGAACACTAGCAATATCAGTTTTTGGTGAGATGGATGTGTCTTCAATTAAA
    CAATTACCAAAAGGTCGTAAACCTATCATTACTACTTGGGCAAAGCATGAGCAATACGAT
    AAAGTTTTGATGCAAATGACCTCAGAGTTGAAAAAAGGTCGTCAAGCATATGTCATTTGC
    CCGCTAATAGAAAGTTCTGAGCATCTCGAAGATGTTCAAAATGTTGTCGCATTGTACGAG
    TCTTTACAACAGTATTATGGTGTTTCCCGTGTAGGGTTATTGCATGGTAAGTTATCTGCC
    GATGAAAAAGATGAGGTCATGCAAAAGTTTAGTAATCATGAGATAAATGTTTTAGTTTCT
    ACTACTGTTGTTGAAGTAGGTGTTAATGTACCGAATGCAACTTTTATGATGATTTATGAT
    GCGGATCGCTTTGGATTATCAACTTTACATCAGTTACGCGGTCGTGTAGGTAGAAGTGAC
    CAGCAAAGTTACTGTGTTTTAATTGCATCCCCTAAAACAGAAACAGGAATTGAAAGAATG
    ACAATTATGACACAAACAACGGATGGATTTGAATTGAGTGAACGAGACTTAGAAATGCGT
    GGTCCTGGAGATTTCTTTGGTGTTAAACAAAGTGGATTGCCAGATTTCTTAGTTGCCAAT
    TTAGTTGAAGATTATCGTATGTTAGAAGTTGCTCGTGATGAAGCAGCTGAACTTATTCAA
    TCTGGCGTATTCTTTGAAAATACGTATCAACATTTACGTCATTTTGTTGAAGAAAATTTA
    TTACATCGTAGTTTTGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2061

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01194
  • symbol: SAOUHSC_01194
  • description: ATP-dependent DNA helicase RecG
  • length: 686
  • theoretical pI: 6.25283
  • theoretical MW: 78342.4
  • GRAVY: -0.268805

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 751.8)
    and 12 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 354)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 56.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 43.8)
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 41.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 39)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 35.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 29.3)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 25.9)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 21.7)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 16.7)
    putative RecB family nuclease, TM0106 family (TIGR03491; HMM-score: 14.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 14.2)
  • TheSEED  :
    • ATP-dependent DNA helicase RecG (EC 3.6.1.-)
    DNA Metabolism DNA replication DNA-replication  ATP-dependent DNA helicase RecG (EC 3.6.1.-)
    and 1 more
    RNA Metabolism Transcription Transcription factors bacterial  ATP-dependent DNA helicase RecG (EC 3.6.1.-)
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 87.4)
    OB (CL0021) RecG_wedge; RecG wedge domain (PF17191; HMM-score: 71.3)
    and 4 more
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 62.6)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 45.6)
    AAA_30; AAA domain (PF13604; HMM-score: 17.9)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 16)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001363
    • TAT(Tat/SPI): 0.000071
    • LIPO(Sec/SPII): 0.000178
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPTRYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFNQPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEGIKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRTYAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEIFRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLMALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITDEQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPIITTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGVSRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGLSTLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGDFFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLHRSFD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

T Niga, H Yoshida, H Hattori, S Nakamura, H Ito
Cloning and sequencing of a novel gene (recG) that affects the quinolone susceptibility of Staphylococcus aureus.
Antimicrob Agents Chemother: 1997, 41(8);1770-4
[PubMed:9257758] [WorldCat.org] [DOI] (P p)

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