PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1120 [new locus tag: SAUSA300_RS06060 ]
• pan locus tag^?: SAUPAN003511000
• symbol: recG
• pan gene symbol^?: recG
• synonym:
• product: ATP-dependent DNA helicase RecG

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1120 [new locus tag: SAUSA300_RS06060 ]
• symbol: recG
• product: ATP-dependent DNA helicase RecG
• replicon: chromosome
• strand: +
• coordinates: 1225776..1227836
• length: 2061
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913866 NCBI
• RefSeq: YP_493817 NCBI
• BioCyc: see SAUSA300_RS06060
• MicrobesOnline: 1292635 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGGCTAAAGTAAACTTAATAGAAAGTCCATATTCTCTTTTACAATTAAAAGGTATAGGT
  CCTAAGAAAATAGAAGTATTGCAACAACTAAATATTCATACAGTGGAAGATCTTGTTCTT
  TATTTGCCAACTAGATATGAAGATAATACAGTGATTGATTTGAATCAAGCAGAAGATCAA
  TCTAACGTTACGATAGAAGGACAAGTATATACAGCTCCAGTAGTTGCATTTTTTGGAAGA
  AATAAATCAAAATTAACCGTTCATTTAATGGTAAATAATATTGCTGTCAAATGTATTTTT
  TTCAATCAACCGTATTTAAAAAAGAAAATCGAATTAAATCAAACTATAACTGTTAAAGGT
  AAGTGGAATAGGGTTAAACAGGAAATTACTGGTAATAGGGTTTTCTTTAATTCACAAGGG
  ACACAAACTCAAGAAAACGCAGATGTTCAATTAGAACCAGTCTATCGTATTAAGGAAGGT
  ATTAAACAAAAGCAAATACGAGACCAAATTAGACAAGCGTTAAATGATGTGACAATTCAT
  GAATGGTTAACTGATGAACTAAGAGAAAAATATAAATTAGAGACCTTGGACTTTACTTTG
  AACACATTACATCATCCTAAAAGTAAAGAGGATTTATTACGTGCTCGTAGAACCTATGCA
  TTTACTGAACTGTTTTTATTCGAATTACGTATGCAATGGCTAAATAGATTAGAAAAGTCA
  TCTGACGAAGCAATTGAAATTGATTATGACATAGACCAAGTTAAATCATTTATTGATCGT
  TTACCTTTTGAACTAACTGAAGCACAGAAATCCAGTGTTAATGAAATTTTTAGAGATTTA
  AAAGCACCAATACGTATGCATCGATTACTTCAAGGTGATGTAGGTTCAGGAAAAACAGTA
  GTTGCTGCAATTTGTATGTATGCGTTAAAAACTGCTGGTTATCAATCAGCATTGATGGTA
  CCAACTGAAATTTTAGCAGAGCAACATGCTGAAAGTTTAATGGCTTTATTTGGAGATTCT
  ATGAACGTTGCATTGTTAACTGGGTCAGTAAAAGGTAAGAAACGAAAGATACTTTTAGAA
  CAACTTGAAAATGGTACGATTGATTGTTTAATTGGAACCCATGCTTTGATTCAAGATGAT
  GTGATTTTCCATAATGTTGGTTTAGTAATTACAGATGAACAACATCGATTTGGTGTGAAT
  CAACGCCAGCTTTTAAGAGAAAAAGGTGCAATGACGAATGTGTTATTTATGACAGCAACG
  CCGATACCAAGAACACTAGCAATATCAGTTTTTGGTGAGATGGATGTGTCTTCAATTAAA
  CAATTACCAAAAGGTCGTAAACCTATCATTACTACTTGGGCAAAGCATGAGCAATACGAT
  AAAGTTTTGATGCAAATGACCTCAGAGTTGAAAAAAGGTCGTCAAGCATATGTCATTTGC
  CCGCTAATAGAAAGTTCTGAGCATCTCGAAGATGTTCAAAATGTTGTCGCATTGTACGAG
  TCTTTACAACAGTATTATGGTGTTTCCCGTGTAGGGTTATTGCATGGTAAGTTATCTGCC
  GATGAAAAAGATGAGGTCATGCAAAAGTTTAGTAATCATGAGATAAATGTTTTAGTTTCT
  ACTACTGTTGTTGAAGTAGGTGTTAATGTACCGAATGCAACTTTTATGATGATTTATGAT
  GCGGATCGCTTTGGATTATCAACTTTACATCAGTTACGCGGTCGTGTAGGTAGAAGTGAC
  CAGCAAAGTTACTGTGTTTTAATTGCATCCCCTAAAACAGAAACAGGAATTGAAAGAATG
  ACAATTATGACACAAACAACGGATGGATTTGAATTGAGTGAACGAGACTTAGAAATGCGT
  GGTCCTGGAGATTTCTTTGGTGTTAAACAAAGTGGATTGCCAGATTTCTTAGTTGCCAAT
  TTAGTTGAAGATTATCGTATGTTAGAAGTTGCTCGTGATGAAGCAGCTGAACTTATTCAA
  TCTGGCGTATTCTTTGAAAATACGTATCAACATTTACGTCATTTTGTTGAAGAAAATTTA
  TTACATCGTAGTTTTGACTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1120 [new locus tag: SAUSA300_RS06060 ]
• symbol: RecG
• description: ATP-dependent DNA helicase RecG
• length: 686
• theoretical pI: 6.25283
• theoretical MW: 78342.4
• GRAVY: -0.268805

⊟Function

• reaction:
  EC 3.6.4.12?  ExPASy
  DNA helicase ATP + H₂O = ADP + phosphate
  EC 3.6.1.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 751.8)
  and 12 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 354)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 56.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 43.8)
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 41.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 39)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 35.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 29.3)
  DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 25.9)
  DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 21.7)
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 16.7)
  putative RecB family nuclease, TM0106 family (TIGR03491; HMM-score: 14.9)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 14.2)
• TheSEED  :
  • ATP-dependent DNA helicase RecG (EC 3.6.1.-)
  DNA Metabolism DNA replication DNA-replication  ATP-dependent DNA helicase RecG (EC 3.6.1.-)
  and 1 more
  RNA Metabolism Transcription Transcription factors bacterial  ATP-dependent DNA helicase RecG (EC 3.6.1.-)
• PFAM:
  P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 87.4)
  OB (CL0021) RecG_wedge; RecG wedge domain (PF17191; HMM-score: 71.3)
  and 4 more
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 62.6)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 45.6)
  AAA_30; AAA domain (PF13604; HMM-score: 17.9)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 16)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001363
  • TAT(Tat/SPI): 0.000071
  • LIPO(Sec/SPII): 0.000178
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161257 NCBI
• RefSeq: YP_493817 NCBI
• UniProt: A0A0H2XIE8 UniProt

⊟Protein sequence

• MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPTRYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFNQPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEGIKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRTYAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEIFRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLMALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITDEQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPIITTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGVSRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGLSTLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGDFFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLHRSFD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications