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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01193
  • pan locus tag?: SAUPAN003509000
  • symbol: SAOUHSC_01193
  • pan gene symbol?: fakA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01193
  • symbol: SAOUHSC_01193
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1143994..1145640
  • length: 1647
  • essential: no DEG other strains

gene symbol: vfrB [1]

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAAT
    TTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGAT
    ACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTG
    TCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGA
    GGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGATTTTGTAAAAATATTGAAAGT
    GAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCA
    TATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCG
    CAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATT
    ATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAA
    GTTGGTGTTGTTGATAGTGGCGGTAAAGGTTTGTTATGCGTTTACGAAGGATTCTTAAAA
    GCGCTTAAAGGTGAAAAAGTTGAAGCCAAAGTTGCAAAGATAGATAAAGATGAATTTGTA
    CATGATGAACATGATTTCCATGGTGTAATTAATACTGAAGATATTATTTATGGCTATTGT
    ACTGAAATGATGGTTCGTTTTGGAAAGAATAAAAAAGCCTTTGATGAACAAGAATTCAGG
    CAAGATATGAGTCAATTTGGTGATTCTTTATTAGTCATTAATGATGAAGAAATTGTGAAA
    GTTCACGTGCATACCGAATACCCAGGTAAAGTGTTTAATTATGGTCAACAATATGGTGAA
    TTAATTAAACTTAAGGTTGAAAATATGAGAGAACAGCACCGTGAAGTGATTCGAAAAGAA
    CAGCACACAGCTAAACCGAAAATGGAAACGGTTGAAACAGCAATTATTACTATTTCTATG
    GGTGAAGGTATTTCAGAGATATTTAAATCAATGGGTGCCACACATATCATTAGTGGTGGA
    CAAACGATGAATCCTTCTACAGAAGATATCGTTAAAGTCATTGAACAATCAAAATGTAAA
    CGTGCAATTATTTTACCGAATAATAAAAATATCTTAATGGCAAGTGAACAAGCAGCGAGT
    ATTGTTGATGCAGAAGCTGTTGTTATTCCAACGAAATCTATTCCTCAAGGTATAAGCGCA
    CTATTCCAATATGATGTGGACGCAACACTTGAAGAAAATAAAGCGCAAATGGCTGATTCA
    GTAAATAACGTTAAATCTGGTTCATTAACGTACGCTGTTCGTGATACGAAAATTGATGGC
    GTTGAGATTAAAAAAGACGCGTTTATGGGCTTGATTGAAGATAAGATTGTAAGCAGCCAA
    AGTGATCAATTAACAACGGTTACTGAGTTGTTAAATGAGATGTTAGCAGAAGATAGTGAA
    ATATTGACTGTGATTATTGGTCAAGATGCAGAGCAAGCAGTTACAGATAACATGATAAAC
    TGGATCGAAGAGCAATATCCAGATGTAGAAGTGGAAGTTCATGAAGGTGGACAACCAATT
    TATCAATATTTCTTTTCAGTAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1647

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01193
  • symbol: SAOUHSC_01193
  • description: hypothetical protein
  • length: 548
  • theoretical pI: 4.3893
  • theoretical MW: 60515.3
  • GRAVY: -0.240146

⊟Function[edit | edit source]

  • TIGRFAM:
    DAK2 domain fusion protein YloV (TIGR03599; HMM-score: 699)
    and 2 more
    dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 38.1)
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 21)
  • TheSEED  :
    • Dihydroxyacetone kinase-like protein, kinase domain
    • Dihydroxyacetone kinase-like protein, phosphatase domain
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Dihydroxyacetone kinase family protein
  • PFAM:
    EDD (CL0245) Dak1_2; Dihydroxyacetone kinase family (PF13684; HMM-score: 399.4)
    and 3 more
    no clan defined Dak2; DAK2 domain (PF02734; HMM-score: 138.7)
    DUF2574; Protein of unknown function (DUF2574) (PF10836; HMM-score: 12.2)
    Hybrid (CL0105) GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022801
    • TAT(Tat/SPI): 0.000565
    • LIPO(Sec/SPII): 0.006205
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTNMNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESESEINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYIIVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDEFVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDEEIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETAIITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNILMASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLTYAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQDAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jeffrey L Bose, Seth M Daly, Pamela R Hall, Kenneth W Bayles
    Identification of the Staphylococcus aureus vfrAB operon, a novel virulence factor regulatory locus.
    Infect Immun: 2014, 82(5);1813-22
    [PubMed:24549328] [WorldCat.org] [DOI] (I p)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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