PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1069 [new locus tag: SA_RS06060 ]
• pan locus tag^?: SAUPAN003509000
• symbol: SA1069
• pan gene symbol^?: fakA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1069 [new locus tag: SA_RS06060 ]
• symbol: SA1069
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1208175..1209821
• length: 1647
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123900 NCBI
• RefSeq: NP_374342 NCBI
• BioCyc: see SA_RS06060
• MicrobesOnline: 103368 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAAT
  TTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGAT
  ACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTG
  TCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGA
  GGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGATTTTGTAAAAATATTGAAAGT
  GAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCA
  TATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCG
  CAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATT
  ATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAA
  GTTGGTGTTGTTGATAGTGGCGGTAAAGGTTTGTTATGCGTTTACGAAGGATTCTTAAAA
  GCGCTTAAAGGTGAAAAAGTTGAAGCCAAAGTTGCAAAGATAGATAAAGATGAATTTGTA
  CATGATGAACATGATTTCCATGGTGTAATTAATACTGAAGATATTATTTATGGCTATTGT
  ACTGAAATGATGGTTCGTTTTGGAAAGAATAAAAAAGCCTTTGATGAACAAGAATTCAGG
  CAAGATATGAGTCAATTTGGTGATTCTTTATTAGTCATTAATGATGAAGAAATTGTGAAA
  GTTCACGTGCATACCGAATACCCAGGTAAAGTGTTTAATTATGGTCAACAATATGGTGAA
  TTAATTAAACTTAAGGTTGAAAATATGAGAGAACAGCACCGTGAAGTGATTCGAAAAGAA
  CAGCACACAGCTAAACCGAAAATGGAAACGGTTGAAACAGCAATTATTACTATTTCTATG
  GGTGAAGGTATTTCAGAGATATTCAAATCAATGGGTGCCACACATATCATTAGTGGTGGA
  CAAACGATGAATCCTTCTACAGAAGATATCGTTAAAGTCATTGAACAATCAAAATGTAAA
  CGTGCAATTATTTTACCGAATAATAAAAATATTTTAATGGCAAGTGAACAAGCAGCGAGT
  ATTGTTGATGCAGAAGCAGTTGTTATTCCAACGAAATCTATTCCTCAAGGTATAAGCGCA
  CTATTCCAATATGATGTGGACGCAACACTTGAAGAAAATAAAGCGCAAATGGCTGATTCA
  GTAAATAACGTTAAATCTGGTTCATTAACGTACGCTGTTCGTGATACGAAAATTGATGGC
  GTTGAGATTAAAAAAGACGCGTTTATGGGCTTGATTGAAGATAAGATTGTAAGCAGCCAA
  AGTGATCAATTAACAACGGTTACTGAGTTGTTAAATGAGATGTTAGCAGAAGATAGTGAA
  ATATTGACTGTGATTATTGGTCAAGATGCAGAGCAAGCAGTTACAGATAACATGATAAAC
  TGGATCGAAGAGCAATATCCAGATGTAGAAGTGGAAGTTCATGAAGGTGGACAACCAATT
  TATCAATATTTCTTTTCAGTAGAATAA
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⊟Protein

⊟General

• locus tag: SA1069 [new locus tag: SA_RS06060 ]
• symbol: SA1069
• description: hypothetical protein
• length: 548
• theoretical pI: 4.3893
• theoretical MW: 60515.3
• GRAVY: -0.240146

⊟Function

• TIGRFAM:
  DAK2 domain fusion protein YloV (TIGR03599; HMM-score: 699)
  and 2 more
  dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 38.1)
  dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 21)
• TheSEED  :
  • Dihydroxyacetone kinase-like protein, kinase domain
  • Dihydroxyacetone kinase-like protein, phosphatase domain
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Dihydroxyacetone kinase family protein
• PFAM:
  EDD (CL0245) Dak1_2; Dihydroxyacetone kinase family (PF13684; HMM-score: 399.4)
  and 3 more
  no clan defined Dak2; DAK2 domain (PF02734; HMM-score: 138.7)
  DUF2574; Protein of unknown function (DUF2574) (PF10836; HMM-score: 12.2)
  Hybrid (CL0105) GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022801
  • TAT(Tat/SPI): 0.000565
  • LIPO(Sec/SPII): 0.006205
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926809 NCBI
• RefSeq: NP_374342 NCBI
• UniProt: Q7A5Z4 UniProt

⊟Protein sequence

• MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTNMNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESESEINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYIIVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDEFVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDEEIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETAIITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNILMASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLTYAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQDAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1068 > SA1069

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications