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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1240 [new locus tag: SACOL_RS06340 ]
  • pan locus tag?: SAUPAN003509000
  • symbol: SACOL1240
  • pan gene symbol?: fakA
  • synonym:
  • product: DAK2 domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1240 [new locus tag: SACOL_RS06340 ]
  • symbol: SACOL1240
  • product: DAK2 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1247760..1249406
  • length: 1647
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAAT
    TTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGAT
    ACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTG
    TCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGA
    GGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGATTTTGTAAAAATATTGAAAGT
    GAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCA
    TATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCG
    CAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATT
    ATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAA
    GTTGGTGTTGTTGATAGTGGCGGTAAAGGTTTGTTATGCGTTTACGAAGGATTCTTAAAA
    GCGCTTAAAGGTGAAAAAGTTGAAGCCAAAGTTGCAAAGATAGATAAAGATGAATTTGTA
    CATGATGAACATGATTTCCATGGTGTAATTAATACTGAAGATATTATTTATGGCTATTGT
    ACTGAAATGATGGTTCGTTTTGGAAAGAATAAAAAAGCCTTTGATGAACAAGAATTCAGG
    CAAGATATGAGTCAATTTGGTGATTCTTTATTAGTCATTAATGATGAAGAAATTGTGAAA
    GTTCACGTGCATACCGAATACCCAGGTAAAGTGTTTAATTATGGTCAACAATATGGTGAA
    TTAATTAAACTTAAGGTTGAAAATATGAGAGAACAGCACCGTGAAGTGATTCGAAAAGAA
    CAGCACACAGCTAAACCGAAAATGGAAACGGTTGAAACAGCAATTATTACTATTTCTATG
    GGTGAAGGTATTTCAGAGATATTTAAATCAATGGGTGCCACACATATCATTAGTGGTGGA
    CAAACGATGAATCCTTCTACAGAAGATATCGTTAAAGTCATTGAACAATCAAAATGTAAA
    CGTGCAATTATTTTACCGAATAATAAAAATATCTTAATGGCAAGTGAACAAGCAGCGAGT
    ATTGTTGATGCAGAAGCTGTTGTTATTCCAACGAAATCTATTCCTCAAGGTATAAGCGCA
    CTATTCCAATATGATGTGGACGCAACACTTGAAGAAAATAAAGCGCAAATGGCTGATTCA
    GTAAATAACGTTAAATCTGGTTCATTAACGTACGCTGTTCGTGATACGAAAATTGATGGC
    GTTGAGATTAAAAAAGACGCGTTTATGGGCTTGATTGAAGATAAGATTGTAAGCAGCCAA
    AGTGATCAATTAACAACGGTTACTGAGTTGTTAAATGAGATGTTAGCAGAAGATAGTGAA
    ATATTGACTGTGATTATTGGTCAAGATGCAGAGCAAGCAGTTACAGATAACATGATAAAC
    TGGATCGAAGAGCAATATCCAGATGTAGAAGTGGAAGTTCATGAAGGTGGACAACCAATT
    TATCAATATTTCTTTTCAGTAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1647

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1240 [new locus tag: SACOL_RS06340 ]
  • symbol: SACOL1240
  • description: DAK2 domain-containing protein
  • length: 548
  • theoretical pI: 4.3893
  • theoretical MW: 60515.3
  • GRAVY: -0.240146

⊟Function[edit | edit source]

  • TIGRFAM:
    DAK2 domain fusion protein YloV (TIGR03599; HMM-score: 699)
    and 2 more
    dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 38.1)
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 21)
  • TheSEED  :
    • Dihydroxyacetone kinase-like protein, kinase domain
    • Dihydroxyacetone kinase-like protein, phosphatase domain
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Dihydroxyacetone kinase family protein
  • PFAM:
    EDD (CL0245) Dak1_2; Dihydroxyacetone kinase family (PF13684; HMM-score: 399.4)
    and 3 more
    no clan defined Dak2; DAK2 domain (PF02734; HMM-score: 138.7)
    DUF2574; Protein of unknown function (DUF2574) (PF10836; HMM-score: 12.2)
    Hybrid (CL0105) GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022801
    • TAT(Tat/SPI): 0.000565
    • LIPO(Sec/SPII): 0.006205
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTNMNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESESEINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYIIVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDEFVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDEEIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETAIITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNILMASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLTYAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQDAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 973 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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