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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS13910 [old locus tag: SACOL2656 ]
- pan locus tag?: SAUPAN006363000
- symbol: SACOL_RS13910
- pan gene symbol?: arcB
- synonym:
- product: ornithine carbamoyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS13910 [old locus tag: SACOL2656 ]
- symbol: SACOL_RS13910
- product: ornithine carbamoyltransferase
- replicon: chromosome
- strand: -
- coordinates: 2717409..2718419
- length: 1011
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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961ATGACAGAAATTCAAAAACCGTATGATTTAAAAGGCAGATCATTATTAAAAGAAAGTGAT
TTTACCAAAGCAGAATTCGAAGGACTTATTGATTTTGCAATTACATTAAAAGAGTATAAG
AAAAACGGTATTAAGCATCACTACTTATCTGGAAAAAATATTGCACTACTATTCGAAAAG
AATTCGACGAGAACGCGTGCTGCGTTTACAGTTGCGTCTATTGATTTAGGTGCGCATCCA
GAATTTTTAGGAAAAAATGATATTCAATTAGGCAAAAAAGAATCTGTAGAGGATACTGCG
AAAGTATTAGGTAGAATGTTCGATGGTATTGAATTCCGTGGTTTTTCACAACAAGCTGTT
GAAGATTTAGCGAAGTTCTCTGGTGTACCGGTGTGGAATGGATTAACAGACGATTGGCAT
CCTACACAAATGTTAGCTGATTTTATGACAATAAAAGAGAATTTTGGATATCTAGAAGGA
ATAAACTTAACTTACGTTGGAGATGGACGTAATAATATTGCGCATTCATTAATGGTAGCA
GGTGCTATGTTAGGTGTTAATGTAAGAATTTGTACACCTAAATCATTAAATCCAAAAGAG
GCATATGTTGATATTGCAAAAGAAAAAGCGAGTCAATATGGTGGTTCAGTCATGATTACG
GATAATATTGCAGAAGCAGTTGAAAATACAGATGCTATATATACAGATGTTTGGGTATCG
ATGGGTGAAGAAAGTGAATTTGAACAACGTATTAATTTATTAAAAGACTATCAAGTGAAT
CAACAGATGTTTGATTTAACAGGTAAAGATTCAACGATATTCTTACATTGTTTACCAGCA
TTCCATGATACAAATACACTTTATGGACAAGAAATTTATGAAAAATATGGATTAGCTGAA
ATGGAAGTTACAGACCAAATCTTTAGAAGTGAACATTCAAAAGTGTTTGATCAAGCTGAA
AATAGAATGCATACAATTAAGGCAGTAATGGCAGCAACATTGGGGAGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS13910 [old locus tag: SACOL2656 ]
- symbol: SACOL_RS13910
- description: ornithine carbamoyltransferase
- length: 336
- theoretical pI: 4.94437
- theoretical MW: 37762.5
- GRAVY: -0.331548
⊟Function[edit | edit source]
- reaction: EC 2.1.3.3? ExPASyOrnithine carbamoyltransferase Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
- TIGRFAM: Amino acid biosynthesis Glutamate family ornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 414)and 3 moreEnergy metabolism Amino acids and amines putrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 261.7)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis aspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 125.5)knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 70.9)
- TheSEED: see SACOL2656
- PFAM: Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 196.1)and 1 moreno clan defined OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 153.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001766
- TAT(Tat/SPI): 0.000557
- LIPO(Sec/SPII): 0.000344
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGIKHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKVLGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEGINLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVMITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLHCLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATLGS
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: see SACOL2656
- quantitative data / protein copy number per cell:
- interaction partners:
SACOL_RS01080 acetyl-CoA acetyltransferase [1] (data from MRSA252) SACOL_RS01085 3-hydroxyacyl-CoA dehydrogenase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArcR*, ArgR, CcpA, Rex* see SACOL2656
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)