PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13410 [old locus tag: SACOL2560 ]
• pan locus tag^?: SAUPAN006206000
• symbol: SACOL_RS13410
• pan gene symbol^?: mvaA
• synonym:
• product: hydroxymethylglutaryl-CoA reductase, degradative

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13410 [old locus tag: SACOL2560 ]
• symbol: SACOL_RS13410
• product: hydroxymethylglutaryl-CoA reductase, degradative
• replicon: chromosome
• strand: -
• coordinates: 2618093..2619370
• length: 1278
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2618093..2619370) NCBI
• BioCyc: SACOL_RS13410 BioCyc
• MicrobesOnline: see SACOL2560

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGCAAAATTTAGATAAGAATTTTCGACATTTATCTCGTAAAGAAAAGTTACAACAATTG
  GTTGATAAGCAATGGTTATCAGAAGAACAATTCGACATTTTACTGAATCATCCATTAATC
  GATGAAGAAGTAGCCAATAGTTTAATTGAAAATGTCATCGCGCAAGGTGCATTACCCGTT
  GGATTATTACCGAATATCATTGTGGACGATAAGGCATATGTTGTACCTATGATGGTGGAA
  GAGCCTTCAGTTGTCGCTGCAGCTAGTTATGGTGCAAAGCTAGTGAATCAGACTGGCGGA
  TTTAAAACGGTATCTTCTGAACGTATTATGATAGGTCAAATCGTCTTTGATGGCGTTGAC
  GATACTGAAAAATTATCAGCAGACATTAAAGCTTTAGAAAAGCAAATTCATAAAATTGCG
  GATGAGGCATATCCTTCTATTAAAGCGCGTGGTGGTGGTTACCAACGTATAGCGATTGAT
  ACATTTCCTGAGCAACAGTTACTATCTTTAAAAGTATTTGTTGATACGAAAGATGCTATG
  GGCGCTAATATGCTTAATACGATTTTAGAGGCCATAACTGCATTTTTAAAAAATGAATTT
  CCGCAAAGCGACATTTTAATGAGTATTTTATCCAATCATGCAACAGCGTCCGTTGTTAAA
  GTTCAAGGCGAAATTGATGTTAAAGATTTAGCAAGGGGCGAGAGAACTGGAGAAGAGGTT
  GCCAAACGAATGGAACGTGCTTCTGTATTGGCCCAAGTAGATATTCATCGTGCAGCAACA
  CATAATAAAGGTGTTATGAATGGCATACATGCTGTTGTTTTAGCAACAGGAAATGATACG
  CGTGGTGCAGAAGCAAGTGCGCATGCATACGCGAGTCGTGACGGACAGTATCGTGGTATT
  GCTACATGGCGTTACGATCAAGATCGTCAACGATTGATTGGTACAATTGAAGTGCCTATG
  ACATTGGCAATCGTTGGCGGTGGTACAAAAGTATTACCAATTGCTAAAGCTTCATTAGAG
  CTACTAAATGTAGAGTCAGCACAAGAATTAGGTCATGTAGTTGCTGCCGTTGGTTTAGCG
  CAAAACTTTGCAGCATGTCGCGCGCTTGTGTCAGAAGGTATTCAACAAGGTCATATGAGT
  TTACAATATAAATCATTAGCTATCGTTGTAGGGGCAAAAGGTGATGAAATTGCTAAAGTA
  GCTGAAGCTTTGAAAAAAGAACCCCGTGCAAATACACAAGCAGCGGAACATATTTTACAA
  GAAATTAGACAACAATAG
  60
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  1140
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  1260
  1278
  

⊟Protein

⊟General

• locus tag: SACOL_RS13410 [old locus tag: SACOL2560 ]
• symbol: SACOL_RS13410
• description: hydroxymethylglutaryl-CoA reductase, degradative
• length: 425
• theoretical pI: 5.98668
• theoretical MW: 46236.6
• GRAVY: -0.109647

⊟Function

• reaction:
  EC 1.1.1.88?  ExPASy
  Hydroxymethylglutaryl-CoA reductase (R)-mevalonate + CoA + 2 NAD⁺ = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
• TIGRFAM:
  Metabolism Energy metabolism Other hydroxymethylglutaryl-CoA reductase, degradative (TIGR00532; EC 1.1.1.88; HMM-score: 335)
  and 2 more
  Metabolism Central intermediary metabolism Other hydroxymethylglutaryl-CoA reductase (NADPH) (TIGR00533; EC 1.1.1.34; HMM-score: 63.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids 3-hydroxy-3-methylglutaryl-coenzyme A reductase (TIGR00920; HMM-score: 53.7)
• TheSEED: see SACOL2560
• PFAM:
  no clan defined HMG-CoA_red; Hydroxymethylglutaryl-coenzyme A reductase (PF00368; HMM-score: 340.5)
  and 3 more
  SLOG (CL0349) YAcAr; YspA, cpYpsA-related SLOG family (PF10686; HMM-score: 13.8)
  no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 13.5)
  AEP (CL0243) PPL4; Prim-pol 4 (PF18188; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.99
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0096
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001125
  • TAT(Tat/SPI): 0.000088
  • LIPO(Sec/SPII): 0.000133
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447103067 NCBI
• RefSeq: WP_001180323 NCBI
• UniProt: see SACOL2560

⊟Additional information (user-provided)

⊟Protein sequence

• MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEVANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFKTVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAIDTFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASVVKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLATGNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPIAKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGAKGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ

⊟Experimental data

• experimentally validated: see SACOL2560
• protein localization: see SACOL2560
• quantitative data / protein copy number per cell: see SACOL2560
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL2560

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications