PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2333 [new locus tag: SA_RS13370 ]
• pan locus tag^?: SAUPAN006206000
• symbol: mvaA
• pan gene symbol^?: mvaA
• synonym:
• product: hydroxymethylglutaryl-CoA reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2333 [new locus tag: SA_RS13370 ]
• symbol: mvaA
• product: hydroxymethylglutaryl-CoA reductase
• replicon: chromosome
• strand: -
• coordinates: 2617222..2618499
• length: 1278
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125260 NCBI
• RefSeq: NP_375657 NCBI
• BioCyc: see SA_RS13370
• MicrobesOnline: 104683 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCAAAGTTTAGATAAGAATTTCCGACATTTATCTCGTCAACAAAAGTTACAACAATTG
  GTAGATAAGCAATGGTTATCAGAAGATCAATTCGACATTTTATTGAATCATCCATTAATT
  GATGAGGAAGTAGCAAATAGTTTAATTGAAAATGTCATCGCGCAAGGTGCATTACCCGTT
  GGATTATTACCGAATATCATTGTGGACGATAAGGCATATGTTGTACCTATGATGGTGGAA
  GAGCCTTCAGTTGTCGCTGCAGCTAGTTATGGTGCAAAGCTAGTGAATCAGACTGGCGGA
  TTTAAAACGGTATCTTCTGAACGTATTATGATAGGTCAAATCGTCTTTGATGGCGTTGAC
  GATACTGAAAAATTATCAGCAGACATTAAAGCTTTAGAAAAGCAAATTCATAAAATTGCG
  GATGAGGCATATCCTTCTATTAAAGCGCGTGGTGGTGGTTACCAACGTATAGCTATTGAT
  ACATTTCCTGAGCAACAGTTACTATCTTTAAAAGTATTTGTTGATACGAAAGATGCTATG
  GGCGCTAATATGCTTAATACGATTTTAGAGGCCATAACTGCATTTTTAAAAAATGAATCT
  CCACAAAGCGACATTTTAATGAGTATTTTATCCAATCATGCAACAGCGTCCGTTGTTAAA
  GTTCAAGGCGAAATTGACGTTAAAGATTTAGCAAGGGGCGAGAGAACTGGAGAAGAGGTT
  GCCAAACGAATGGAACGTGCTTCTGTATTGGCACAAGTTGATATTCATCGTGCTGCAACA
  CATAATAAAGGTGTTATGAATGGCATACATGCCGTTGTTTTAGCAACAGGAAATGATACG
  CGTGGTGCAGAAGCAAGTGCGCATGCATACGCGAGTCGTGACGGACAGTATCGTGGTATT
  GCAACATGGAGATACGATCAAAAACGTCAACGTTTAATTGGTACAATAGAAGTGCCTATG
  ACATTGGCAATCGTTGGCGGTGGTACAAAAGTATTACCAATTGCTAAAGCTTCTTTAGAA
  TTGCTAAATGTAGATTCAGCACAAGAATTAGGTCATGTAGTTGCTGCCGTTGGTTTAGCA
  CAGAACTTTGCAGCATGTCGCGCGCTCGTTTCCGAAGGTATCCAGCAAGGCCATATGAGC
  TTGCAATATAAATCTTTAGCTATTGTTGTAGGTGCAAAAGGTGATGAAATTGCGCAAGTA
  GCTGAAGCATTGAAGCAAGAACCCCGTGCGAATACACAAGTAGCTGAACGCATTTTACAA
  GAAATTAGACAACAATAG
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⊟Protein

⊟General

• locus tag: SA2333 [new locus tag: SA_RS13370 ]
• symbol: MvaA
• description: hydroxymethylglutaryl-CoA reductase
• length: 425
• theoretical pI: 6.02407
• theoretical MW: 46180.5
• GRAVY: -0.107294

⊟Function

• reaction:
  EC 1.1.1.88?  ExPASy
  Hydroxymethylglutaryl-CoA reductase (R)-mevalonate + CoA + 2 NAD⁺ = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
• TIGRFAM:
  Metabolism Energy metabolism Other hydroxymethylglutaryl-CoA reductase, degradative (TIGR00532; EC 1.1.1.88; HMM-score: 330.2)
  and 2 more
  Metabolism Central intermediary metabolism Other hydroxymethylglutaryl-CoA reductase (NADPH) (TIGR00533; EC 1.1.1.34; HMM-score: 61.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids 3-hydroxy-3-methylglutaryl-coenzyme A reductase (TIGR00920; HMM-score: 49.7)
• TheSEED  :
  • Hydroxymethylglutaryl-CoA reductase (NADPH) (EC 1.1.1.34)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)
  and 1 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)
• PFAM:
  no clan defined HMG-CoA_red; Hydroxymethylglutaryl-coenzyme A reductase (PF00368; HMM-score: 338)
  and 2 more
  UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 16)
  Co_AT_N; Cob(I)alamin adenosyltransferase N terminal (PF12557; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000944
  • TAT(Tat/SPI): 0.000088
  • LIPO(Sec/SPII): 0.000123
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928124 NCBI
• RefSeq: NP_375657 NCBI
• UniProt: A0A0H3JW54 UniProt

⊟Protein sequence

• MQSLDKNFRHLSRQQKLQQLVDKQWLSEDQFDILLNHPLIDEEVANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFKTVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAIDTFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNESPQSDILMSILSNHATASVVKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLATGNDTRGAEASAHAYASRDGQYRGIATWRYDQKRQRLIGTIEVPMTLAIVGGGTKVLPIAKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGAKGDEIAQVAEALKQEPRANTQVAERILQEIRQQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications