PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13185 [old locus tag: SACOL2518 ]
• pan locus tag^?: SAUPAN006134000
• symbol: SACOL_RS13185
• pan gene symbol^?: relP
• synonym:
• product: GTP pyrophosphokinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13185 [old locus tag: SACOL2518 ]
• symbol: SACOL_RS13185
• product: GTP pyrophosphokinase
• replicon: chromosome
• strand: -
• coordinates: 2577779..2578471
• length: 693
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2577779..2578471) NCBI
• BioCyc: SACOL_RS13185 BioCyc
• MicrobesOnline: see SACOL2518

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTATGTAGATCGAAAACCATCACTATATTTAGAGGATTTGCGACATGATTTTAAAAAT
  AGTTTAAGTAAATTTGAAAATGGTGATGAAGCATTTGATACGTTATTAGGTTTCGTAGAG
  TTAGATCATATTTATTCGTCAGCACTAAAGGAAATAAGCACTAAACTGAGTATTTTAGAT
  GACAATTTCAATCACATTTATAAACACAATCCTATACATCATATGGAGCGACGTGTGAAA
  GAAATGCGTAGTTTAATAGAAAAGCTTAATCGTAAAGGATTACAGATTAGCGCAGAAACT
  GCCAAAGAACATATACTGGATATTGCCGGAATTCGCGTAGTATGTAATTACTTAGATGAT
  ATTTATTTGATTGAAGAGATGTTGCTTAAACAAGAAGACGTACAATTGATAAAACGTAAA
  GATTATATTCAGCACCCTAAAGAAAATGGTTACCGCAGTTTACATATCGTTGTATCTATT
  CCAGTCTTTTTAGCAGAACGCGTTGAGGTATTGCCTGTTGAAATTCAAATTAGAACGATA
  GGTATGGATATGTGGGCAAGTTTAGAACATAAAATACGTTATAAAAACAATGCAGAGACG
  GAAAAGTATCGAGATTTACTGAAAGAATGTGCGACAGAGATTACTGAAGTTGAAGATAAA
  TTACAACAAATTCATTCTGAAATAACAGAGTAG
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⊟Protein

⊟General

• locus tag: SACOL_RS13185 [old locus tag: SACOL2518 ]
• symbol: SACOL_RS13185
• description: GTP pyrophosphokinase
• length: 230
• theoretical pI: 5.77612
• theoretical MW: 27165
• GRAVY: -0.47087

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 59.7)
• TheSEED: see SACOL2518
• PFAM:
  NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 120.7)
  and 5 more
  SNARE-fusion (CL0445) V-SNARE; Vesicle transport v-SNARE protein N-terminus (PF05008; HMM-score: 19.1)
  HRDC-like (CL0426) UvsW-1; UvsW.1 domain (PF11637; HMM-score: 14.3)
  no clan defined DUF4140; N-terminal domain of unknown function (DUF4140) (PF13600; HMM-score: 13.7)
  GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 13.1)
  HTH (CL0123) RNase_H2-Ydr279; Ydr279p protein family (RNase H2 complex component) wHTH domain (PF09468; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9787
  • Cytoplasmic Membrane Score: 0.0116
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0096
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00153
  • TAT(Tat/SPI): 0.000146
  • LIPO(Sec/SPII): 0.000279
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446211281 NCBI
• RefSeq: WP_000289136 NCBI
• UniProt: see SACOL2518

⊟Protein sequence

• MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHIYSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAKEHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSIPVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVEDKLQQIHSEITE

⊟Experimental data

• experimentally validated: see SACOL2518
• protein localization: see SACOL2518
• quantitative data / protein copy number per cell: see SACOL2518
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL2518

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Tobias Geiger, Benjamin Kästle, Fabio Lino Gratani, Christiane Goerke, Christiane Wolz
Two small (p)ppGpp synthases in Staphylococcus aureus mediate tolerance against cell envelope stress conditions.
J Bacteriol: 2014, 196(4);894-902
[PubMed:24336937] [WorldCat.org] [DOI] (I p)