From AureoWiki

Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL_RS12650 [old locus tag: SACOL2411 ]

pan locus tag^?: SAUPAN005947000

symbol: SACOL_RS12650

pan gene symbol^?: tcyB

synonym:
product: amino acid ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS12650 [old locus tag: SACOL2411 ]
symbol: SACOL_RS12650
product: amino acid ABC transporter permease
replicon: chromosome
strand: -
coordinates: 2470616..2471335
length: 720
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (2470616..2471335) NCBI
BioCyc: SACOL_RS12650 BioCyc
MicrobesOnline: see SACOL2411

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
ATGTTTCTAAATCTAAATAGTGAACAACAACATGCGCTAGATGCTGCAAAACAAGCTTTC
GGACCTATGCTAGAAGGTTTGGTCAAATATTCAATTCCTATTACATTAGTTACATTTGTT
TTAGGATTGATTATTGCATTATTTACAGCATTAATGCGAATTTCAACGAGTAAAATTTTA
AGAAGTATTTCACGTGTCTATGTATCTATTATTCGAGGAACACCAATGATAGTACAACTA
TTTATCATATTTTATGGTATTCCAGAATTAGGTAGATTATTAACAAATGACGCTGACAAC
CAATGGACATTGGCACCTGTAGTGGCTGCTATTATTGGTTTATCATTAAATGTAGGTGCG
TATGCTTCGGAAATTATTCGTGGCGGTATTATTTCTATACCGAAAGGACAAACAGAAGCT
GCATATTCCATCGGTATGACGTATGGTCAAACGATACAACGTATCATTTTACCGCAGGCA
ATTCGAGTGTCGATTCCTGCACTAGGTAATACATTTTTAAGTTTAATCAAAGATACATCA
TTATTAGGATTTATTTTAGTGGCTGAAATGTTTAGAAAAGCTCAAGAAGTTGCGTCTACA
ACATATGAATATTTAACAATTTATGTGTTAGTTGCGCTAATGTACTGGGTGGTATGCTTT
ATTATTTCAATTATCCAAGGTATCTATGAATCTTATATTGAAAGAGGGTATCGCTCATGA
60
120
180
240
300
360
420
480
540
600
660
720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL_RS12650 [old locus tag: SACOL2411 ]
symbol: SACOL_RS12650
description: amino acid ABC transporter permease
length: 239
theoretical pI: 9.13859
theoretical MW: 26442.2
GRAVY: 0.682427

⊟Function[edit | edit source]

⊞TIGRFAM:
ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 159.4)
ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 138.3)
and 7 more
Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 84)
Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 23.7)
Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 20.9)
Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 17.5)
Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthetic protein FliQ (TIGR01402; HMM-score: 15.1)
Cellular processes Cellular processes Pathogenesis type III secretion protein, HrpO family (TIGR01403; HMM-score: 4.8)
Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion protein, HrpO family (TIGR01403; HMM-score: 4.8)
TheSEED: see SACOL2411
⊞PFAM:
BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 95.5)
and 6 more
no clan defined Bac_export_3; Bacterial export proteins, family 3 (PF01313; HMM-score: 16.1)
WSS_VP; White spot syndrome virus structural envelope protein VP (PF12175; HMM-score: 11.9)
DUF3377; Domain of unknown function (DUF3377) (PF11857; HMM-score: 11)
Oxa1 (CL0376) EMC3_TMCO1; Integral membrane protein EMC3/TMCO1-like (PF01956; HMM-score: 8.7)
no clan defined NEMP; NEMP family (PF10225; HMM-score: 7.6)
TSPAN_4TM-like (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
⊞
DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 1
- Cell wall & surface Score: 0
- Extracellular Score: 0
LocateP:
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004341
- TAT(Tat/SPI): 0.000217
- LIPO(Sec/SPII): 0.001597
predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

GI: 446401721 NCBI
RefSeq: WP_000479576 NCBI
UniProt: see SACOL2411

⊟Protein sequence[edit | edit source]

MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLIIALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQWTLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQAIRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVVCFIISIIQGIYESYIERGYRS

⊟Experimental data[edit | edit source]

experimentally validated: see SACOL2411
protein localization: see SACOL2411
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CymR* see SACOL2411

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS12650&oldid=75721"