PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS10670 [old locus tag: SACOL2043 ]
• pan locus tag^?: SAUPAN005304000
• symbol: SACOL_RS10670
• pan gene symbol^?: ilvB
• synonym:
• product: acetolactate synthase, large subunit, biosynthetic type

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS10670 [old locus tag: SACOL2043 ]
• symbol: SACOL_RS10670
• product: acetolactate synthase, large subunit, biosynthetic type
• replicon: chromosome
• strand: +
• coordinates: 2102785..2104554
• length: 1770
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2102785..2104554) NCBI
• BioCyc: SACOL_RS10670 BioCyc
• MicrobesOnline: see SACOL2043

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTAAAACTCAACATGAAGTAAACCAAAATATTGACCCTTTAAAAATGGCTGAATCA
  CTTGAACCTGAACAACTAAATGAAAAAACTTTAAATGATATGCGTTCAGGATCAGAAGTG
  CTAGTAGAAGCTCTACTTAAAGAAAATGTGGATTATTTATTCGGTTATCCTGGTGGTGCC
  GTACTACCTTTATATGACACGTTTTATGATGGTAAAATCAAACATATTTTAGCAAGACAC
  GAACAAGGTGCTGTTCATGCTGCAGAAGGTTATGCACGTGTATCTGGTAAAACTGGCGTC
  GTTGTAGTTACAAGCGGTCCAGGTGCAACTAATGTAATGACAGGTATTACGGATGCACAT
  TGCGACTCTTTACCTCTAGTTGTATTCACTGGACAAGTTGCTACACCAGGCATTGGTAAA
  GATGCATTCCAAGAAGCGGATATTCTATCTATGACTTCACCAATTACAAAACAAAATTAT
  CAAGTGAAACGTGTTGAAGATATCCCTAAAATCGTACACGAAGCTTTCCATGTAGCTAAT
  TCTGGACGCAAAGGTCCTGTAGTGATTGATTTTCCAAAAGATATGGGTGTTTTAGCTACA
  AATGTGGATTTATGCGACGAAATCAATATTCCAGGTTATGAAGTTGTTACAGAACCAGAA
  AATAAAGACATTGACACTTTCATCTCACTTTTAAAAGAAGCGAAAAAGCCTGTCGTATTA
  GCCGGCGCAGGTATTAATCAATCAAAATCAAATCAATTATTAACACAGTTTGTTAATAAA
  CATCAAATCCCAACAGTTACTACTTTACTAGGTCTAGGTGCTGTACCTTATGAGGATACA
  CTATTTTTAGGTATGGGAGGAATGCATGGTTCTTATGCTAGTAACATGGCATTAACTGAG
  TGTGATTTACTCATTAATTTAGGTAGCCGCTTCGATGATAGATTAGCAAGCAAACCTGAT
  GCCTTTGCACCTAACGCCAAAATTGTACATGTAGATATTGATCCTTCAGAAATCAATAAA
  GTTATTCATGTAGATTTAGGTATTATTGCAGACTGTAAAAGATTTTTAGAATGTTTAAAT
  GATAAAAATGTTGAGACTATAGAACACAGTGACTGGGTTAAACATTGTCAAAATAATAAG
  CAGAAACACCCATTTAAACTTGGTGAAGAAGATCAAGTATTTTGTAAGCCACAACAAACA
  ATCGAATATATCGGCAAAATTACAAATGGTGAAGCAATTGTTACTACAGACGTGGGACAA
  CATCAAATGTGGGCAGCTCAATTTTATCCATTTAAAAATCACGGACAATGGGTTACAAGC
  GGTGGTTTAGGAACAATGGGATTCGGTATTCCTTCGTCAATTGGTGCCAAATTAGCTAAT
  CCTGATAAAACAGTCGTATGTTTCGTCGGTGACGGTGGTTTCCAAATGACAAACCAAGAA
  ATGGCACTTTTACCCGAATATGGTTTAGATGTCAAAATCGTACTAATCAATAATGGAACA
  TTAGGTATGGTTAAACAATGGCAAGATAAGTTCTTTAATCAACGCTTCTCACACTCAGTA
  TTTAATGGTCAACCTGATTTTATGAAAATGGCAGAAGCATATGGCGTCAAAGGTTTCTTA
  ATCGATAAGCCAGAACAACTGGAAGAACAATTAGATGCAGCGTTTGCTTATCAAGGACCA
  GCTTTAATTGAGGTTCGTATTTCCCCTACTGAAGCTGTAACCCCAATGGTTCCGAGTGGC
  AAATCAAATCATGAAATGGAGGGCTTATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS10670 [old locus tag: SACOL2043 ]
• symbol: SACOL_RS10670
• description: acetolactate synthase, large subunit, biosynthetic type
• length: 589
• theoretical pI: 5.02481
• theoretical MW: 64491.1
• GRAVY: -0.201528

⊟Function

• reaction:
  EC 2.2.1.6?  ExPASy
  Acetolactate synthase 2 pyruvate = 2-acetolactate + CO₂
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 744.1)
  and 13 more
  glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 322.9)
  Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 305)
  Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 283.9)
  Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 279.2)
  Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 219.3)
  Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 188.8)
  indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 122.4)
  Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 81.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 58.3)
  phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 39.7)
  sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 30.9)
  2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 16)
  Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 12.1)
• TheSEED: see SACOL2043
• PFAM:
  THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 195.2)
  TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 176.7)
  and 3 more
  FAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 145.1)
  THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 14.8)
  XFP_N; XFP N-terminal domain (PF09364; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, thiamine diphosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005517
  • TAT(Tat/SPI): 0.00026
  • LIPO(Sec/SPII): 0.000345
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445969965 NCBI
• RefSeq: WP_000047820 NCBI
• UniProt: see SACOL2043

⊟Protein sequence

• MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVRISPTEAVTPMVPSGKSNHEMEGL

⊟Experimental data

• experimentally validated: see SACOL2043
• protein localization: see SACOL2043
• quantitative data / protein copy number per cell: see SACOL2043
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SACOL2043

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL2043

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications