PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS07905 [old locus tag: SACOL1552 ]
pan locus tag^?: SAUPAN004044000
symbol: SACOL_RS07905
pan gene symbol^?: malR
• synonym:
• product: LacI family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07905 [old locus tag: SACOL1552 ]
• symbol: SACOL_RS07905
• product: LacI family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 1588495..1589514
• length: 1020
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1588495..1589514) NCBI
• BioCyc: SACOL_RS07905 BioCyc
• MicrobesOnline: see SACOL1552

⊟Phenotype

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⊟DNA sequence

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  ATGGTTACGATTAAAGATGTTGCACTAAAAGCCGGTGTTTCTCCTTCGACAGTTTCAAGA
  GTTATAAAAGGAAATCAACGTATTAGCGAAGCGACAATTTCAAAAGTGAAGAAAGTTATG
  GAAGAATTGAATTATTTTCCTAATACCGCTGCTAGAACTTTAATTACAAACCAAACATAT
  AAAATTGGTTTAGTGTTAAAAGGGTCTGAGGAGCCTATTCGACTGAATCCATTCTACATC
  AATGTATTGCTAGGAATTTCTGAAACGTGTAACCAGCATGGCTATGGTACACAAACGACA
  GTCTCAAATAATATGAATGATTTAATGGATGAAGTTTATAAAATGATTAAACAACGAATG
  GTTGATGCGTTTATACTGCTCTATTCAAAAGAAAATGATCCGATTAAACAAATGTTAATT
  GATGAAAGCATGCCATTTATTGTGATTGGTAAGCCTACATCGGATATAGATCATCAATTT
  ACACATATAGATAATGATAATATATTAGCTTCTGAAAATTTGACACGACATGTTATTGAA
  CAAGGTGTAGATGAATTAATATTTATTACAGAAAAAGGAAATTTTGAAGTTTCAAAAGAT
  AGAATTCAAGGATTTGAAACGATAGCTTCTCAAAATAAAATTAATTATCAAATTATTGAG
  ACTAGTAATGAAAGAGAAGTTATTTTTAATTACATGCAAAATCTACATACGCGTTTGAAG
  GACCCAAATATTAAACAGGCAATCATTTCGTTAGATGCTATGTTACATTTAGCGATTTTA
  AGTGTCCTATATGAACTTAATATTGAAATTCCGAAAGATGTAATGACAGCAACGTTCAAT
  GATTCTTATTTAACCGAAATTGCGTCACCACCACAAACCTGTATTGACATCAAGCCTCGA
  ATGTTAGGTCAACAGGCTGGTGCAGCAATTTTAAATATATTGAAAAACAAAGCACAGGAT
  GTTATTGAACTCGTCATTATAGATACAGAATTAAAAATAAGAAAATCAACACAGCGATAG
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⊟Protein

⊟General

• locus tag: SACOL_RS07905 [old locus tag: SACOL1552 ]
• symbol: SACOL_RS07905
• description: LacI family transcriptional regulator
• length: 339
• theoretical pI: 5.7117
• theoretical MW: 38390.1
• GRAVY: -0.179056

⊟Function

• ⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 117.9)
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 111.6)
  and 5 more
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 50.9)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
  Hypothetical proteins Conserved TIGR02147 family protein (TIGR02147; HMM-score: 14.8)
• TheSEED: see SACOL1552
• ⊞PFAM:
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 76.2)
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 69.4)
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 62.3)
  and 10 more
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 26.7)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 17.1)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 16.9)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.3)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.3)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 14)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 13.1)
  DUF6953; Family of unknown function (DUF6953) (PF22266; HMM-score: 12.8)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 11.9)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by MalR, TF important in Maltose utilization, Maltodextrin utilization: see SACOL1552

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9691
  • Cytoplasmic Membrane Score: 0.019
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0116
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011903
  • TAT(Tat/SPI): 0.002449
  • LIPO(Sec/SPII): 0.00132
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446178483 NCBI
• RefSeq: WP_000256338 NCBI
• UniProt: see SACOL1552

⊟Protein sequence

• MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELNYFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVSNNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQFTHIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQIIETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIRKSTQR

⊟Experimental data

• experimentally validated: see SACOL1552
• protein localization: see SACOL1552
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, MalR see SACOL1552

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications