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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • pan locus tag?: SAUPAN003915000
  • symbol: SACOL_RS07615
  • pan gene symbol?: dinG
  • synonym:
  • product: ATP-dependent helicase DinG

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • symbol: SACOL_RS07615
  • product: ATP-dependent helicase DinG
  • replicon: chromosome
  • strand: -
  • coordinates: 1534589..1537282
  • length: 2694
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1534589..1537282) NCBI
  • BioCyc: SACOL_RS07615 BioCyc
  • MicrobesOnline: see SACOL1495

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
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    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2581
    2641
    ATGGGTATGGCAACCTATGCCGTTGTGGATTTGGAAACAACAGGCAACCAATTAGATTTT
    GACGATATCATTCAAATTGGTATAACGTTTGTGCGCAATAACCAAATTATTGATACATAT
    CATTCTATGATTAGAACAAATTTAGAGATTCCGCCGTTCATTCAAGCGTTAACATCCATT
    GAAGAAAATATGCTACAACAAGCGCCATATTTTAATCAAGTTGCACAAGAAATCTACGAT
    AAGATAAAAGATTGCATTTTTGTAGCTCATAATGTAGATTTTGATTTGAATTTTATTAAA
    AAGGCATTTAAAGATTGTAATATTCAATATCGTCCCAAAAAAGTTATAGATACACTAGAA
    ATCTTTAAAATAGCTTTTCCTACAGATAAAAGCTATCAATTAAGTGAATTAGCAGAGGCA
    CATGGTATTACCTTAGCAAATGCCCATCGAGCTGACGAAGATGCCGCTACTACTGCTAAA
    TTGATGATATTAGCTTTTGAAAAATTTGAAAAGTTACCGTTAGATACTTTGAAGCAATTA
    TATTATTTAAGTAAACAGTTGAAATACGATTTGTATGATATTTTCTTTGAGATGGTTAGG
    CAATATGATGCAAAGCCATTGGATAAATCGTACGAAAAGTTCGAACAGATTATCTATCGT
    AAGCAAGTTGATTTTAAAAAGCCGACAACAAATTATAATGGCAGCTTGAAGTCTTTATAT
    AGCAAAGCTGTAGATCAACTTGGCTTAACATATCGACCGCAACAATTATATTTAGCGGAA
    ACTATATTAGATCAGCTCATGCATAGTGAAAAAGCAATGATTGAAGCATCACTAGGCAGT
    GGTAAATCATTAGCATATTTACTTGCGGCCTTGATGTATAATATTGAAACTGGGAAGCAT
    GTCATGATTTCTACCAATACTAAATTACTGCAAAGTCAATTGTTAGAAAAAGATATTCCA
    GCAATGAATGAAGCGTTGAATTTTAAAATTAATGCACTATTGATTAAAAGTAAAAGTGAT
    TACATTTCATTGGGGCTTATTAGTCAAATTTTAAAAGATGACACAAGTAATTACGAAGTG
    AATATCTTGAAAATGCAATTGCTGATTTGGATTACAGAGACGCCGTCCGGAGATATACAG
    GAATTAAATTTAAAAGGTGGTCAAAAGATGTATTTTGACCAGAAAATTGAAACATATGTA
    CCGGCACGTCACGATGTTCATTATTATAATTTTATTAAGCGAAATGCACAGAACATTCAA
    ATTGGTATTACAAATCATGCACATTTAATTCATTCTGATGTTGAAAATTCAATATATCAA
    TTGTTTGATGATTGTATAGTGGATGAAGCACATCGCTTACCAGACTATGCCTTAAATCAA
    GTGACAAATGAGTTGAGTTATGCTGATATTAAATATCAGCTTGGTTTGATTGGTAAAAAC
    GAAAATGAAAAATTGTTAAAGGCAATTGATCAACTGGAAAAGCAGCGAATTTTGGAAAAA
    TTAGATATTGCACCAATTGATATTTTTGGCTTGAAGGCTAGTATGAATGAGATTCATGAG
    TTAAATGAGCAATTGTTCTCAACGATATTTACGATTATCAATGATTCTGATGTTTATGAT
    GATGATATTCATCGCTTCCATAATGTATTTACTTTTGAAACAAAAGACATTTTGAAGGAT
    TTACATGCGATTATCGATAAGTTAAATAAAACGCTAGAAATATTCAATGGCATTAGTCAT
    AAAACAGTTAAATCATTACGGAAACAGTTGTTGTATTTAAAAGATAAATTTAAAAATATC
    GAACAAAGTTTAAAAGCTGGACATACTAGTTTCATTTCGATTAAAAATTTATCTCAAAAA
    TCAACAATACGTTTATATGTGAAAGATTACGCTGTAAAAGATGTATTAACGAAACAAGTA
    TTGGAAAAATTTAAATCACTCATTTTTATATCTGGTACTTTAAAATTTAATCATTCGTTT
    GAAGCTTTTAAACAGTTATTCAACAAAGATGTTCACTTTAATACATTTGAAGTTAACACG
    TCGTTACAAAGTGCAAAAAATACAAGTGTATTTATACCGAGTGATGTAGCATCTTATCAA
    TATAAAAATATAGATGAGTATGTAGCATCGATTGTAAGCTATATTATTGAATATACGACT
    ATAACGTCATCAAAATGTTTAGTCTTATTTACGAGTTATAAAATGATGCATATGGTACAA
    GATATGCTAAATGAATTGCCAGAATTTGAAGATTATGTTGTATTAACACAACAGCAGAAC
    CAAAATTATAAAATAGTACAACAGTTTAATAATTTTGATAAGGCCATACTGCTTGGTACG
    TCAACATTTTTTGAAGGTTTTGATTTTCAAGCAAATGGTATTAAATGTGTGATGATAGCG
    AAGTTACCGTTTATGAACAAGCATAATGCAAAATATTGGCTTATGGATTCTGAATTCACT
    TCAACTTTCAAGGAATATGTATTACCTGATGCAGTTACACGTTTTAGACAAGGACTTGGA
    AGATTAATTAGAAATGAAAATGATCGCGGCATAATAGTTTCATTTGATGATCGACTCATA
    AACAGTAATTATAAAAACTTTTTTGAACAAACACTTGAAAATTATCGTCAGAAAAAAGGC
    GACATTCAACAGTTTGGAAAATTATTAAGACAAATTCAAAAAAAGAAAAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    720
    780
    840
    900
    960
    1020
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    1140
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    1260
    1320
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    1560
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    2160
    2220
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    2340
    2400
    2460
    2520
    2580
    2640
    2694

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • symbol: SACOL_RS07615
  • description: ATP-dependent helicase DinG
  • length: 897
  • theoretical pI: 6.83206
  • theoretical MW: 104217
  • GRAVY: -0.297101

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 1269.1)
    and 6 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 139.1)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 94.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 60.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair helicase (rad3) (TIGR00604; EC 3.6.4.-; HMM-score: 57.1)
    CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 47.5)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 17.9)
  • TheSEED: see SACOL1495
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C_2; Helicase C-terminal domain (PF13307; HMM-score: 112.3)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 91.3)
    and 5 more
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 22.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 20.7)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 18.7)
    no clan defined HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.6)
    DUF615; Protein of unknown function (DUF615) (PF04751; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002601
    • TAT(Tat/SPI): 0.00012
    • LIPO(Sec/SPII): 0.000314
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMIRTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKAFKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAKLMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQIIYRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEASLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALLIKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYFDQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEAHRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDIFGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIIDKLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1495
  • protein localization: see SACOL1495
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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