From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2615 [new locus tag: SACOL_RS13680 ]
  • pan locus tag?: SAUPAN006293000
  • symbol: panB
  • pan gene symbol?: panB
  • synonym:
  • product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2615 [new locus tag: SACOL_RS13680 ]
  • symbol: panB
  • product: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2671595..2672413
  • length: 819
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    TTGAAAACAGTAAGTCAATTAATAGATATGAAACAAAAGGAAACTAAAATTTCTATGGTA
    ACAGCTTATGATTTTCCAAGTGCTAAACAAGTGGAAGCAGCGGGTATTGATATGATTCTT
    GTTGGGGATTCACTTGGTATGACTGTATTAGGTTATGAAAGTACCGTACAAGTGACGTTA
    GCAGATATGATTCATCACGGTCGTGCAGTAAGAAGAGGCGCACCAAATACATTTGTAGTT
    GTTGATATGCCGATTGGTGCAGTAGGCATCTCTATGACACAAGACTTAAATCATGCTCTA
    AAGCTTTATCAGGAGACGAATGCAAATGCAATTAAAGCAGAAGGAGCGCATATTACACCA
    TTTATTGAAAAAGCAACGGCAATTGGTATTCCTGTTGTTGCACATTTAGGATTAACACCT
    CAAAGTGTTGGCGTTATGGGGTATAAATTACAAGGTGCAACGAAAGAAGCAGCGGAGCAA
    CTTATTTTAGACGCGAAAAATGTAGAACAAGCTGGTGCAGTTGCGTTAGTACTTGAAGCA
    ATTCCAAATGATTTAGCAGAAGAAATTAGCAAGCACTTAACAATTCCAGTCATTGGTATT
    GGTGCAGGAAAAGGTACAGATGGTCAAGTATTGGTTTATCACGATATGTTAAATTATGGT
    GTTGAACATAAAGCGAAATTTGTGAAGCAATTTGCTGATTTTAGTGTTGGTGTGGATGGT
    TTAAAACAATACGATCAAGAAGTTAAATCTGGTGCATTTCCATCAGAAGAATATACGTAT
    AAAAAGAAAATTATGAATGAGGTTAATAACAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2615 [new locus tag: SACOL_RS13680 ]
  • symbol: PanB
  • description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  • length: 272
  • theoretical pI: 5.58058
  • theoretical MW: 29256.4
  • GRAVY: -0.0161765

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.11?  ExPASy
    3-methyl-2-oxobutanoate hydroxymethyltransferase 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 3-methyl-2-oxobutanoate hydroxymethyltransferase (TIGR00222; EC 2.1.2.11; HMM-score: 297.5)
    and 1 more
    Metabolism Energy metabolism Amino acids and amines aspartate racemase (TIGR00035; EC 5.1.1.13; HMM-score: 13.6)
  • TheSEED  :
    • 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
  • PFAM:
    PK_TIM (CL0151) Pantoate_transf; Ketopantoate hydroxymethyltransferase (PF02548; HMM-score: 324.4)
    and 3 more
    PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 19.4)
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 15.6)
    Leu-IlvD (CL0364) PA; PA domain (PF02225; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015916
    • TAT(Tat/SPI): 0.000741
    • LIPO(Sec/SPII): 0.000677
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTVSQLIDMKQKETKISMVTAYDFPSAKQVEAAGIDMILVGDSLGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKLYQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQLILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLNYGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 677 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 20.8 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2615&oldid=70804"