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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman

locus tag: NWMN_2496 [new locus tag: NWMN_RS14325 ]

pan locus tag^?: SAUPAN006293000

symbol: panB

pan gene symbol^?: panB

synonym:
product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_2496 [new locus tag: NWMN_RS14325 ]
symbol: panB
product: 3-methyl-2-oxobutanoate hydroxymethyltransferase
replicon: chromosome
strand: -
coordinates: 2741011..2741829
length: 819
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 5332237 NCBI
RefSeq: YP_001333530 NCBI
BioCyc:
MicrobesOnline: 3708098 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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121
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541
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661
721
781
TTGAAAACAGTAAGTCAATTAATAGATATGAAACAAAAGCAAACTAAAATTTCTATGGTA
ACAGCTTATGATTTTCCAAGTGCTAAACAAGTGGAAGCAGCGGGTATTGATATGATTCTT
GTTGGGGATTCACTTGGTATGACTGTATTAGGTTATGAAAGTATCGTACAAGTGACGTTA
GCAGATATGATTCATCACGGTCGTGCAGTAAGAAGAGGCGCACCAAATACATTTGTAGTT
GTTGATATGCCGATTGGTGCAGTAGGCATCTCTATGACACAAGACTTAAATCATGCTCTA
AAGCTTTATCAGGAGACGAATGCAAATGCAATTAAAGCAGAAGGAGCGCATATTACACCA
TTTATTGAAAAAGCAACGGCAATTGGTATTCCTGTTGTTGCACATTTAGGATTAACACCT
CAAAGTGTTGGCGTTATGGGGTATAAATTACAAGGTGCAACGAAAGAAGCAGCGGAGCAA
CTTATTTTAGACGCGAAAAATGTAGAACAAGCTGGTGCAGTTGCGTTAGTACTTGAAGCA
ATTCCAAATGATTTAGCAGAAGAAATTAGCAAGCACTTAACAATTCCAGTCATTGGTATT
GGTGCAGGAAAAGGTACAGATGGTCAAGTATTGGTTTATCACGATATGTTAAATTATGGT
GTTGAACATAAAGCGAAATTTGTGAAGCAATTTGCTGATTTTAGTGTTGGTGTGGATGGT
TTAAAACAATACGATCAAGAAGTTAAATCTGGTGCATTTCCATCAGAAGAATATACGTAT
AAAAAGAAAATTATGAATGAGGTTAATAACAATGACTAA
60
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540
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819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_2496 [new locus tag: NWMN_RS14325 ]
symbol: PanB
description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
length: 272
theoretical pI: 5.84619
theoretical MW: 29267.5
GRAVY: 0.00294118

⊟Function[edit | edit source]

⊞reaction:
EC 2.1.2.11? ExPASy
3-methyl-2-oxobutanoate hydroxymethyltransferase 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H₂O = tetrahydrofolate + 2-dehydropantoate
⊞TIGRFAM:
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 3-methyl-2-oxobutanoate hydroxymethyltransferase (TIGR00222; EC 2.1.2.11; HMM-score: 294.2)
and 1 more
Metabolism Energy metabolism Amino acids and amines aspartate racemase (TIGR00035; EC 5.1.1.13; HMM-score: 13.7)
⊞
TheSEED :
- 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
and 1 more
Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11)
⊞PFAM:
TIM_barrel (CL0036) Pantoate_transf; Ketopantoate hydroxymethyltransferase (PF02548; HMM-score: 325.2)
and 4 more
PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 18.1)
Asp-glut_race (CL0399) Asp_Glu_race; Asp/Glu/Hydantoin racemase (PF01177; HMM-score: 17.3)
TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 14.5)
Leu-IlvD (CL0364) PA; PA domain (PF02225; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Mg²⁺
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9496
- Cytoplasmic Membrane Score: 0.0386
- Cell wall & surface Score: 0.0004
- Extracellular Score: 0.0115
⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.019194
- TAT(Tat/SPI): 0.000656
- LIPO(Sec/SPII): 0.000597
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 151222708 NCBI
RefSeq: YP_001333530 NCBI
UniProt: A6QK86 UniProt

⊟Protein sequence[edit | edit source]

MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDSLGMTVLGYESIVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKLYQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQLILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLNYGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: panD < panC < panB

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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