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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1868 [new locus tag: SACOL_RS09580 ]
  • pan locus tag?: SAUPAN004566000
  • symbol: splB
  • pan gene symbol?: splB
  • synonym:
  • product: serine protease SplB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1868 [new locus tag: SACOL_RS09580 ]
  • symbol: splB
  • product: serine protease SplB
  • replicon: chromosome
  • strand: -
  • coordinates: 1919938..1920660
  • length: 723
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAACAAAAACGTAGTCATCAAGAGTTTAGCAGCATTAACAATTTTAACATCTGTAACA
    GGTATTGGAACAACATTGGTTGAGGAAGTACAACAAACTGCCAAAGCAGAAAATAATGTC
    ACAAAAGTTAAAGATACTAATATTTTTCCATATACTGGTGTAGTTGCTTTTAAAAGTGCA
    ACTGGATTTGTAGTTGGAAAGAATACTATTTTAACAAATAAACATGTGTCGAAAAATTAC
    AAAGTGGGCGATCGTATTACTGCACATCCAAATAGTGATAAAGGTAATGGTGGTATTTAT
    TCGATTAAAAAGATTATTAATTATCCAGGTAAAGAAGATGTATCAGTCATTCAAGTTGAA
    GAGCGTGCAATAGAACGTGGACCAAAAGGCTTTAATTTTAATGATAATGTAACGCCATTC
    AAATATGCGGCAGGGGCTAAAGCTGGTGAGCGAATTAAAGTGATTGGTTATCCACACCCA
    TACAAAAATAAATATGTTTTATATGAGTCAACTGGCCCTGTGATGTCAGTAGAAGGTAGC
    AGTATTGTATATTCAGCGCATACTGAAAGCGGAAACTCTGGATCACCTGTATTAAACAGC
    AACAACGAATTAGTTGGTATTCATTTTGCTTCTGATGTAAAAAATGATGATAACAGAAAT
    GCATATGGCGTCTACTTTACACCAGAAATTAAAAAATTCATTGCAGAAAACATAGATAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    723

⊟Protein[edit | edit source]

Protein Data Bank: 2VID

⊟General[edit | edit source]

  • locus tag: SACOL1868 [new locus tag: SACOL_RS09580 ]
  • symbol: SplB
  • description: serine protease SplB
  • length: 240
  • theoretical pI: 9.68796
  • theoretical MW: 26096.3
  • GRAVY: -0.359167

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 18.5)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 18.5)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 16.7)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 16.7)
  • TheSEED  :
    • serine protease
  • ⊞PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 92.5)
    and 6 more
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 64.8)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 25.8)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 23.7)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 22)
    Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 20.3)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.0032
    • Extracellular Score: 0.9968
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.947043
    • TAT(Tat/SPI): 0.008645
    • LIPO(Sec/SPII): 0.012863
    • Cleavage Site: CS pos: 36-37. AKA-EN. Pr: 0.8283
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVKDTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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