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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1568 [new locus tag: SACOL_RS07985 ]
  • pan locus tag?: SAUPAN004078000
  • symbol: xseA
  • pan gene symbol?: xseA
  • synonym:
  • product: exodeoxyribonuclease VII large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1568 [new locus tag: SACOL_RS07985 ]
  • symbol: xseA
  • product: exodeoxyribonuclease VII large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1603767..1605104
  • length: 1338
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAGATTATTTAAGTGTTTCAGCTTTAACGAAATATATTAAATATAAATTTGATCAA
    GATCCACATCTCCAATCTGTATTGATTAAAGGTGAGCTTTCAAATTTCAAAAAGCATTCA
    AGTGGTCATTTATACTTTAATGTGAAAGATAAAGAAAGCGTTATAAGTGCCATGATGTTC
    AAAGGTAGTGCTTCTAAATTAAACTTCGAACCTAAAGAAGGAGATGAAGTCTTATTAGAA
    GCACGTGTTTCTGTTTTTGAACGTCGTGGAAACTATCAAATTTATGTAAATAAAATGCAA
    TTAGATGGTATAGGGAACTTATATCAAAAATTAGAAGCATTAAAGAAAAAATTAACTGAA
    GAAGGTTGTTTTGATAAAGCGAATAAGAAATCAATACCTAAGTTTCCTAAAAAAATTGCA
    GTTTTAACAGCGAGTACAGGTGCCGCAATTCGAGATATCCATTCAACGATAAATAGTCGT
    TTTCCATTAGCTGAACAAATACAAATTAGTACTTTGGTTCAAGGTGAAAAAGCAAAAGAC
    GACATTATTGAAAAAATTGAATATGCAGATAGTTTAGGTGTAGATACCATTATTGTAGGT
    CGAGGCGGTGGATCCATAGAAGATTTATGGAATTTCAACGAAGAAGCTGTCGTCCGTGCA
    ATATATAATTGTAAGACACCGATTATATCAGCAGTTGGTCATGAAACAGACTTTACATTA
    AGTGATTTTGCTGCAGACATCAGAGCTGCGACTCCAACTCAAGCTGCTGTTATTGCAACA
    CCAGACCAATATGAATTGCTGCAACAAATACAGCAATATCAGTTTACATTGACTCGTTTC
    ATCAAGAAACATTTAGAACAACAACGTAAACATGTTGAACATTTGTCATCGTATTACAAG
    TTTAAACAACCAACTTTATTGTATGATCAACAGATACAACGTCGAGATGATTTAGAAAAG
    AGACTGAAACAACAAATTCAGGCAACTTTTGAACAACAGAGACATCGATTAATGCTGTTG
    CAACAACGTTATAATTTAAAAGCATTACTAAGTAGTGTTAATCAAGAACAACAAAATAAT
    CTTCAATTGACAAACCAACTTGTTAAATTATTGAATTCTAAGATATTAAGTTATAAAAAT
    GATTTGAAAAATAAAGTAGAAAACTTAAACAATTTAAGTCCAACTAATACAATGTTGCGT
    GGATATGCAATTGTTAATAAAAAAGACGAAGTCATTACGAGTACTAAAGATTTAACAGAA
    AATGATCAATTGACGTTAACAATGAAAGATGGCTTAGTAGATGCAAAAGTTACGAAAGTA
    AGGTGTAATAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1568 [new locus tag: SACOL_RS07985 ]
  • symbol: XseA
  • description: exodeoxyribonuclease VII large subunit
  • length: 445
  • theoretical pI: 9.48419
  • theoretical MW: 50893.8
  • GRAVY: -0.52809

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.11.6?  ExPASy
    Exodeoxyribonuclease VII Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
  • TIGRFAM:
    Genetic information processing DNA metabolism Degradation of DNA exodeoxyribonuclease VII, large subunit (TIGR00237; EC 3.1.11.6; HMM-score: 489)
  • TheSEED  :
    • Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)
    DNA Metabolism DNA repair DNA repair, bacterial  Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)
  • PFAM:
    no clan defined Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 311.8)
    and 7 more
    OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 96.8)
    tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 34.4)
    Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 16.6)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 12.3)
    PepSY_TM-like (CL0490) PepSY_TM_like_2; Putative PepSY_TM-like (PF16357; HMM-score: 11.8)
    no clan defined Transglut_i_TM; Inactive transglutaminase fused to 7 transmembrane helices (PF14400; HMM-score: 11.3)
    DUF4279; Domain of unknown function (DUF4279) (PF14106; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002793
    • TAT(Tat/SPI): 0.000164
    • LIPO(Sec/SPII): 0.000268
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 49 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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