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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004078000
- symbol?: xseA
- synonym:
- description?: exodeoxyribonuclease VII large subunit
- exodeoxyribonuclease VII large subunit
- exodeoxyribonuclease 7 large subunit
- exodeoxyribonuclease VII, large subunit, putative
- exodeoxyribonuclease VII, large subunit
- putative exodeoxyribonuclease VII large subunit
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4359689..4361026
- synteny block?: BlockID0031100
- occurrence?: in 100% of 34 strains
xseA : exodeoxyribonuclease VII large subunit [1]
Staphylococci require degradation of single-stranded DNA for multiple cellular processes including DNA mismatch repair, homologous recombination and antiviral defenses. The XseAB system (ExoVII) is a bi-directional (3'→5' or 5'→3') exoDNase that can degrade single-stranded DNA. Typically, incorrect base pairing during replication is detected by MutS which recruits the MutL repair center protein. Unlike in E. coli which uses a separate nickase enzyme (MutH), staphylococcal MutL has a separate domain to nick the unmethylated strand at its hemimethylated GATC recognition site. DNA is unwound, typically by PcrA helicase, and, depending on the location of the nick, a 3'→5' or 5'→3' ssDNA exodeoxyribonuclease is required to depolymerize the non-methylated strand. While RecJ can perform 5'→3' processing, only ExoVII can degrade ssDNA in either orientation.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1482
08BA02176:
C248_1566
11819-97:
MS7_1541 (xseA)
6850:
RSAU_001390 (xseA)
71193:
ST398NM01_1589
ECT-R 2:
ECTR2_1375 (xseA)
ED133:
SAOV_1524 (xseA)
ED98:
SAAV_1516 (xseA)
HO 5096 0412:
SAEMRSA15_14440
JH1:
SaurJH1_1615 (xseA)
JH9:
SaurJH9_1582 (xseA)
JKD6008:
SAA6008_01493 (xseA)
JKD6159:
SAA6159_01459 (xseA)
JSNZ:
JSNZ_001514 (xseA)
LGA251:
SARLGA251_14300
M013:
M013TW_1539
MRSA252:
SAR1601 (xseA)
MSHR1132:
SAMSHR1132_13640
MSSA476:
SAS1462 (xseA)
Mu3:
SAHV_1511 (xseA)
Mu50:
SAV1523 (xseA)
MW2:
MW1476 (xseA)
RF122:
SAB1396c (xseA)
ST398:
SAPIG1589 (xseA)
T0131:
SAT0131_01617 (gltD)
TCH60:
HMPREF0772_11617 (xseA)
TW20:
SATW20_15200
USA300_TCH1516:
USA300HOU_1525 (xseA)
VC40:
SAVC_06865 (xseA)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMF
N315 MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMF
NCTC8325 MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMF
Newman MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMF
USA300_FPR3757 MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMF
************************************************************
COL KGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTE
N315 KGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTE
NCTC8325 KGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTE
Newman KGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTE
USA300_FPR3757 KGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTE
************************************************************
COL EGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKD
N315 EGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKD
NCTC8325 EGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKD
Newman EGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKD
USA300_FPR3757 EGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKD
************************************************************
COL DIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTL
N315 DIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTL
NCTC8325 DIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTL
Newman DIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTL
USA300_FPR3757 DIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTL
************************************************************
COL SDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYK
N315 SDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYK
NCTC8325 SDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYK
Newman SDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYK
USA300_FPR3757 SDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYK
************************************************************
COL FKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNN
N315 FKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNN
NCTC8325 FKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNN
Newman FKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNN
USA300_FPR3757 FKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNN
************************************************************
COL LQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTE
N315 LQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTE
NCTC8325 LQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTE
Newman LQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTE
USA300_FPR3757 LQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTE
************************************************************
COL NDQLTLTMKDGLVDAKVTKVRCNND
N315 NDQLTLTMKDGLVDAKVTKVRCNND
NCTC8325 NDQLTLTMKDGLVDAKVTKVRCNND
Newman NDQLTLTMKDGLVDAKVTKVRCNND
USA300_FPR3757 NDQLTLTMKDGLVDAKVTKVRCNND
*************************
- ↑ Katarzyna Poleszak, Katarzyna H Kaminska, Stanislaw Dunin-Horkawicz, Andrei Lupas, Krzysztof J Skowronek, Janusz M Bujnicki
Delineation of structural domains and identification of functionally important residues in DNA repair enzyme exonuclease VII.
Nucleic Acids Res: 2012, 40(16);8163-74
[PubMed:22718974] [WorldCat.org] [DOI] (I p)Susan T Lovett
The DNA Exonucleases of Escherichia coli.
EcoSal Plus: 2011, 4(2);
[PubMed:26442508] [WorldCat.org] [DOI] (P p)Liping Liu, Hanne Ingmer, Martin Vestergaard
Genome-Wide Identification of Resveratrol Intrinsic Resistance Determinants in Staphylococcus aureus.
Antibiotics (Basel): 2021, 10(1);
[PubMed:33467002] [WorldCat.org] [DOI] (P e)