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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1465 [new locus tag: SACOL_RS07475 ]
  • pan locus tag?: SAUPAN003861000
  • symbol: SACOL1465
  • pan gene symbol?: cvfC
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1465 [new locus tag: SACOL_RS07475 ]
  • symbol: SACOL1465
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1476298..1477422
  • length: 1125
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAAATTTTACGTATAGAGCCAACACCAAGTCCAAATACAATGAAAGTTGTTTTGTCA
    TATACAAGAGAAGACAAGTTATCTAATACTTATAAAAAAGTAGAAGAAACACAACCAAGA
    TTTATAAATCAGTTGTTATCTATAGATGGTATCACTTCCATTTTTCATGTCATGAACTTC
    TTAGCTGTTGATAAGGCACCAAAAGCTGATTGGGAAGTCATATTACCTGATATTAAAGCT
    GCTTTTTCTGATGCGAATAAGGTTTTAGAATCTGTAAATGAACCTCAAATTGACAATCAT
    TTTGGTGAAATTAAAGCTGAATTATTAACTTTTAAGGGTATACCGTATCAAATTAAGCTA
    ACTTCTGCTGACCAAGAATTAAGAGAACAATTACCACAAACATATGTTGACCATATGACT
    CAAGCGCAAACAGCACATGACAATATTGTTTTTATGCGTAAATGGCTAGATTTAGGAAAT
    CGCTATGGAAATATTCAAGAAGTAATGGATGGTGTCCTAGAAGAAGTGCTAGCTACCTAT
    CCAGAATCACAGTTACCCGTATTGGTAAAACATGCTTTAGAAGAAAATCACGCAACTAAT
    AATTATCATTTCTATCGACATGTCTCTTTGGATGAATATCATGCAACTGATAATTGGAAG
    ACTCGATTACGAATGTTAAACCATTTTCCAAAGCCGACTTTTGAAGATATACCGCTGCTT
    GATTTAGCTTTATCTGATGAAAAAGTACCGGTTAGACGTCAAGCGATTGTATTATTAGGT
    ATGATTGAAAGTAAAGAAATTTTACCGTATTTATATAAGGGGCTTCGTGATAAAAGTCCT
    GCTGTAAGAAGAACAGCAGGGGATTGCATAAGCGATTTAGGGTATCCAGAGGCACTACCA
    GAAATGGTGCTACTATTAGATGATCCACAGAAAATCGTTAGGTGGCGTGCTGCTATGTTT
    ATCTTTGATGAAGGTAATGCAGAGCAGCTTCCCGCACTAAAAGCCCATATTAATGACAAT
    GCGTTTGAAGTTAAATTACAAATTGAAATGGCCATATCTCGTATTGAAAACGGAGATGAA
    GCACTTGGTTCAGTATGGAAACAAATGGCAAACAGAACAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1465 [new locus tag: SACOL_RS07475 ]
  • symbol: SACOL1465
  • description: hypothetical protein
  • length: 374
  • theoretical pI: 5.09121
  • theoretical MW: 42922.9
  • GRAVY: -0.338235

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • PBS lyase HEAT-like repeat domain protein
  • PFAM:
    no clan defined Virulence_fact; Virulence factor (PF13769; HMM-score: 89)
    and 8 more
    Nfu_N; Scaffold protein Nfu/NifU N terminal (PF08712; HMM-score: 64.4)
    TPR (CL0020) HEAT_2; HEAT repeats (PF13646; HMM-score: 53.8)
    HEAT_EZ; HEAT-like repeat (PF13513; HMM-score: 32.6)
    HEAT; HEAT repeat (PF02985; HMM-score: 32.4)
    Cnd1; non-SMC mitotic condensation complex subunit 1 (PF12717; HMM-score: 27.1)
    HEAT_PBS; PBS lyase HEAT-like repeat (PF03130; HMM-score: 17.3)
    no clan defined DUF4088; Protein of unknown function (DUF4088) (PF13317; HMM-score: 12.5)
    TPR (CL0020) Adaptin_N; Adaptin N terminal region (PF01602; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003062
    • TAT(Tat/SPI): 0.00031
    • LIPO(Sec/SPII): 0.000715
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQLLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFGEIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGNRYGNIQEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDNWKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLRDKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALKAHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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