From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1377 [new locus tag: SACOL_RS07010 ]
  • pan locus tag?: SAUPAN003717000
  • symbol: tkt
  • pan gene symbol?: tkt
  • synonym:
  • product: transketolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1377 [new locus tag: SACOL_RS07010 ]
  • symbol: tkt
  • product: transketolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1380327..1382315
  • length: 1989
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTTAATGAAAAAGATCAATTAGCTGTTGATACGCTACGTGCACTAAGTATCGACACA
    ATCGAAAAAGCGAATTCTGGTCATCCAGGATTACCTATGGGAGCTGCCCCAATGGCTTAC
    ACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTAAAGATTACTTCAATAGAGAC
    CGTTTCGTATTATCTGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTACATGTTTCT
    GGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAAACACCAGGT
    CATCCTGAATACAGACATACAGATGGTGTAGAAGTTACTACCGGACCACTTGGACAAGGT
    TTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACCTAGCAGGGAAATTTAATAAA
    GAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGTGATTTAATG
    GAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGTAAATTAGTT
    GTTTTATACGATTCAAATGATATTTCATTAGATGGCGAATTAAACAAAGCTTTTTCTGAA
    AACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACTTACTAGTTAAAGATGGTAAT
    GATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGACCAACGATT
    ATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACTAATGGTGTT
    CATGGGGCACCTTTAGGTGAAGTTGAAAGAAAATTAACATTCGAAAATTACGGTTTAGAT
    CCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAATTTTCCAAAATACTATGTTA
    AAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATATGCAGAAACA
    TATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTAT
    AAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGT
    ACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTT
    GCTGGTTCAAACAAATCCAATGTAAATGATGCAACTGATTATAGTTCTGAAACACCTGAA
    GGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTAAATGGTATG
    GCTGCACATGGAGGTTTACATCCATATGGTGCAACATTCTTCGTATTTAGTGATTATTTA
    AAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATGCAACGTTCATCTTCACACAT
    GATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAGCAATTAGCTGGA
    TTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTA
    GCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAA
    AACTTACCGGTATTAGATGTACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCC
    TATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAA
    GTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCAGTACGTGTT
    GTTTCAATGCCTAACTGGAATGCATTTGAACAACAATCTGAAGAATATAAAGAATCAGTT
    ATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGGCTTCACCGCTTGGATGGCAT
    AAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCAAGTGCACCT
    GGCGATTTAGTAGTTGAAAAATATGGATTTACAAAAGAAAATATCTTAAACCAAGTTATG
    AGCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1989

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1377 [new locus tag: SACOL_RS07010 ]
  • symbol: Tkt
  • description: transketolase
  • length: 662
  • theoretical pI: 4.70733
  • theoretical MW: 72250.3
  • GRAVY: -0.332326

⊟Function[edit | edit source]

  • reaction:
    EC 2.2.1.1?  ExPASy
    Transketolase Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Pentose phosphate pathway transketolase (TIGR00232; EC 2.2.1.1; HMM-score: 915.2)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 34.3)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 20.8)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 17.6)
    alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 15.6)
  • TheSEED  :
    • Transketolase (EC 2.2.1.1)
    Carbohydrates Central carbohydrate metabolism Pentose phosphate pathway  Transketolase (EC 2.2.1.1)
  • PFAM:
    THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 466.1)
    and 5 more
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 135.1)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 38.5)
    THDP-binding (CL0254) DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 25.3)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 17.7)
    E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Ca2+, Co2+, Mg2+, Mn2+, thiamine diphosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028852
    • TAT(Tat/SPI): 0.011648
    • LIPO(Sec/SPII): 0.00233
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLMEGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4] [5]
  • quantitative data / protein copy number per cell: 8928 [6]
  • interaction partners:
    SACOL1741(icd)isocitrate dehydrogenase  [7] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [7] (data from MRSA252)
    SACOL0588(rpoB)DNA-directed RNA polymerase subunit beta  [7] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [7] (data from MRSA252)
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [7] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [7] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [7] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 29.94 h [8]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  8. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1377&oldid=78284"