From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1177 [new locus tag: SA_RS06690 ]
  • pan locus tag?: SAUPAN003717000
  • symbol: tkt
  • pan gene symbol?: tkt
  • synonym:
  • product: transketolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1177 [new locus tag: SA_RS06690 ]
  • symbol: tkt
  • product: transketolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1338627..1340615
  • length: 1989
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTTAATGAAAAAGATCAATTAGCTGTTGATACGCTACGTGCACTAAGTATCGACACA
    ATCGAAAAAGCGAATTCTGGTCATCCAGGATTACCTATGGGAGCTGCCCCAATGGCTTAC
    ACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTAAAGATTACTTCAATAGAGAC
    CGTTTCGTATTATCGGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTACATGTTTCT
    GGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAAACACCAGGC
    CATCCTGAATACAGACATACTGATGGTGTAGAAGTTACTACTGGACCACTTGGACAAGGT
    TTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACCTAGCAGGGAAATTTAATAAA
    GAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGTGATTTAATG
    GAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGTAAATTAGTT
    GTTTTATACGATTCAAATGATATTTCATTAGATGGCGAATTAAACAAAGCTTTTTCTGAA
    AACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACTTACTAGTTAAAGATGGTAAT
    GATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGACCAACGATT
    ATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACTAATGGTGTT
    CATGGGGCACCTTTAGGTGAAGTTGAAAGAAAATTAACATTCGAAAATTACGGTTTAGAT
    CCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAATTTTCCAAAATACTATGTTA
    AAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATATGCAGAAACA
    TATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTAT
    AAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGT
    ACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTT
    GCTGGTTCAAACAAATCCAATGTAAATGATGCAACAGATTATAGTTCTGAAACACCTGAA
    GGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTAAATGGTATG
    GCTGCACATGGAGGTTTACATCCATATGGTGCAACATTCTTCGTATTTAGTGATTATTTA
    AAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATGCAACGTTCATCTTCACACAT
    GATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAACAATTAGCTGGA
    TTAAGAGCTATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTA
    GCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAA
    AACTTACCGGTTTTAGATGTACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCC
    TATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAA
    GTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCAGTGCGTGTT
    GTTTCAATGCCTAACTGGAATGCATTTGAACAACAATCTGAAGAATATAAAGAATCAGTT
    ATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGGCTTCACCGCTTGGATGGCAT
    AAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCAAGTGCACCT
    GGCGATTTAGTAGTTGAAAAATATGGATTTACTAAAGAAAATATCTTAAACCAAGTTATG
    AGCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1989

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1177 [new locus tag: SA_RS06690 ]
  • symbol: Tkt
  • description: transketolase
  • length: 662
  • theoretical pI: 4.70733
  • theoretical MW: 72250.3
  • GRAVY: -0.332326

⊟Function[edit | edit source]

  • reaction:
    EC 2.2.1.1?  ExPASy
    Transketolase Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Pentose phosphate pathway transketolase (TIGR00232; EC 2.2.1.1; HMM-score: 915.2)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 34.3)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 20.8)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 17.6)
    alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 15.6)
  • TheSEED  :
    • Transketolase (EC 2.2.1.1)
    Carbohydrates Central carbohydrate metabolism Pentose phosphate pathway  Transketolase (EC 2.2.1.1)
  • PFAM:
    THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 466.1)
    and 5 more
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 135.1)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 38.5)
    THDP-binding (CL0254) DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 25.3)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 17.7)
    E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Ca2+, Co2+, Mg2+, Mn2+, thiamine diphosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028852
    • TAT(Tat/SPI): 0.011648
    • LIPO(Sec/SPII): 0.00233
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLMEGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1517(citC)isocitrate dehydrogenase  [2] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [2] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [2] (data from MRSA252)
    SA0500(rpoB)DNA-directed RNA polymerase subunit beta  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0627hypothetical protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1177&oldid=83879"