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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0538 [new locus tag: SACOL_RS02750 ]
  • pan locus tag?: SAUPAN002233000
  • symbol: ipk
  • pan gene symbol?: ipk
  • synonym:
  • product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0538 [new locus tag: SACOL_RS02750 ]
  • symbol: ipk
  • product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 547101..547949
  • length: 849
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGATATATGAAACGGCACCAGCCAAAATTAATTTTACGCTCGATACACTTTTTAAAAGA
    AATGATGGCTATCATGAGATTGAAATGATAATGACAACAGTTGATTTAAATGATCGTTTA
    ACTTTTCATAAAAGAAAAGATCGAAAGATAGTTGTTGAGATTGAACATAATTATGTGCCT
    TCTAATCATAAAAATCTCGCATATCGTGCAGCGCAACTATTTATTGAGCAATATCAACTA
    AAGCAAGGTGTAACAATTTCTATCGATAAAGAAATACCTGTTTCTGCTGGCTTAGCTGGA
    GGTTCGGCTGATGCAGCAGCAACGTTAAGAGGATTGAATCGACTTTTTGATATAGGGGCG
    AGTTTGGAAGAATTGGCTCTACTAGGCAGTAAAATCGGGACAGATATTCCGTTTTGTATT
    TATAATAAAACTGCACTATGTACTGGAAGAGGAGAGAAAATCGAGTTTTTAAATAAACCA
    CCTTCAGCTTGGGTGATTCTTGCTAAACCAAACTTAGGCATATCATCACCAGATATATTT
    AAGTTGATTAATTTAGATAAGCGTTACGACGTACATACGAAAATGTGTTATGAGGCCTTA
    GAAAATCGAGATTATCAACAATTATGTCAAAGTTTGTCTAATCGATTAGAGCCAATTTCT
    GTTTCAAAACACCCACAAATCGATAAATTAAAAAATAATATGTTGAAAAGTGGTGCAGAT
    GGTGCGTTAATGAGTGGAAGCGGACCTACTGTGTATGGGCTAGCACGAAAAGAAAGCCAA
    GCAAAAAATATTTATAATGCAGTTAACGGTTGTTGTAATGAAGTGTACTTAGTTAGACTA
    TTAGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0538 [new locus tag: SACOL_RS02750 ]
  • symbol: Ipk
  • description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
  • length: 282
  • theoretical pI: 8.54156
  • theoretical MW: 31442
  • GRAVY: -0.251064

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.148?  ExPASy
    4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 297.4)
    and 5 more
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 52.3)
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 39)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 35.2)
    Unknown function Enzymes of unknown specificity beta-ribofuranosylaminobenzene 5'-phosphate synthase family (TIGR00144; HMM-score: 13.9)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 12.3)
  • TheSEED  :
    • Diphosphocytidyl-sugar kinase
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148)
  • PFAM:
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 44.5)
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 44.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001934
    • TAT(Tat/SPI): 0.000105
    • LIPO(Sec/SPII): 0.000429
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHKRKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGSADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKPPSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEPISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVYLVRLLG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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