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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0404 [new locus tag: SACOL_RS02035 ]
  • pan locus tag?: SAUPAN001868000
  • symbol: SACOL0404
  • pan gene symbol?: mepR
  • synonym:
  • product: MarR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0404 [new locus tag: SACOL_RS02035 ]
  • symbol: SACOL0404
  • product: MarR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 410848..411267
  • length: 420
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGAATTCACTTATTCGTATTTATTTAGAATGATTAGTCATGAGATGAAACAAAAGGCT
    GATCAAAAGTTAGAGCAATTTGATATTACAAATGAGCAAGGTCATACGTTAGGTTATCTT
    TATGCACATCAACAAGATGGACTGACACAAAATGATATTGCTAAAGCATTACAACGAACA
    GGTCCAACTGTCAGTAATTTATTAAGGAACCTTGAACGTAAAAAGCTGATCTATCGCTAT
    GTCGATGCACAAGATACGAGAAGAAAGAATATAGGGCTGACTACCTCTGGGATTAAACTC
    GTAGAAGCATTCACTTCGATATTTGATGAAATGGAACAAACACTCGTATCGCAGTTATCT
    GAAGAAGAAAATGAACAAATGAAAGCAAACTTAACTAAAATGTTATCTAGTTTACAATAA
    60
    120
    180
    240
    300
    360
    420

⊟Protein[edit | edit source]

Protein Data Bank: 5F6F
Protein Data Bank: 5FFX

⊟General[edit | edit source]

  • locus tag: SACOL0404 [new locus tag: SACOL_RS02035 ]
  • symbol: SACOL0404
  • description: MarR family transcriptional regulator
  • length: 139
  • theoretical pI: 6.26722
  • theoretical MW: 16172.2
  • GRAVY: -0.669784

⊟Function[edit | edit source]

  • TIGRFAM:
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 40.9)
    and 5 more
    mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 30)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 22.8)
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 20.4)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 13.8)
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 13.6)
  • TheSEED  :
    • Transcriptional regulator, MarR family
  • PFAM:
    HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 38.6)
    MarR; MarR family (PF01047; HMM-score: 38.1)
    and 19 more
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 22.9)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 21.2)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 20.3)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 18.4)
    HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 17.8)
    no clan defined DUF445; Protein of unknown function (DUF445) (PF04286; HMM-score: 17.6)
    HTH (CL0123) HxlR; HxlR-like helix-turn-helix (PF01638; HMM-score: 17.2)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.2)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 16.8)
    B-block_TFIIIC; B-block binding subunit of TFIIIC (PF04182; HMM-score: 16.5)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.3)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 15.2)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 15.2)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14.4)
    DUF1670; Protein of unknown function (DUF1670) (PF07900; HMM-score: 14.3)
    Crp; Bacterial regulatory proteins, crp family (PF00325; HMM-score: 13.1)
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 12.7)
    no clan defined Vps4_C; Vps4 C terminal oligomerisation domain (PF09336; HMM-score: 12.4)
    HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: Bis-indoles; Distamycin
  • genes regulated by MepR*, TF important in Multidrug resistanceRegPrecise
    repression
    mepR*
    mepA*

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005476
    • TAT(Tat/SPI): 0.000487
    • LIPO(Sec/SPII): 0.000763
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQQDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVEAFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MepR* (repression) regulon
    MepR*(TF)important in Multidrug resistance; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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