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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1974 [new locus tag: SA_RS11370 ]
  • pan locus tag?: SAUPAN005560000
  • symbol: SA1974
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1974 [new locus tag: SA_RS11370 ]
  • symbol: SA1974
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2242102..2243289
  • length: 1188
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCTAGATAAAAATCAATTAGCTAAATATAAACAAGATCATTTGTGTGAATATGAAAAA
    ATAATGAGTAACAATGAAAAAGAAGCACTTGAAGAGAAGGTAGCGTCATTAGATTTAGAT
    TTTATTGCCAAATTATATAACGATTTATACATCAATAAGAAAACAATAGATGATGTATCT
    GCAGTTTCTGAAGTGAAATATGATATTAAATCACAAATGAGTGATGATGAGATTAAGCGT
    TTAGAGGAACAAGGACTTCAAGCTATTAAAGAAGGACAATTTGCAGTACTTTTGATGGCA
    GGTGGTCAAGGAACAAGACTTGGTTACAAGGGCCCTAAAGGTTCGTTTGAAATTGAAGGT
    GTAAGTTTATTTGAACTTCAAGCTAACCAATTAAAAACATTGAATCATCAATCTGGACAT
    ACGATTCAATGGTATATTATGACAAGTGATATCAATCATGAAGAAACTTTAGCTTATTTC
    GAAGCACATAGTTATTTTGGATATGATCAAGAAGCAATCCATTTCTTTAAACAAGATAAT
    ATTGTGGCGCTTAGTGAAGAAGGGAAGTTAATTTTAAATCAACAGGGTCGTATTATGGAA
    ACGCCGAATGGTAATGGTGGCGTGTTTAAATCTTTAGACAAGGCAGGATACTTAGAAGAA
    ATGTCTAATAATGGCGTTAAGTATATTTTCTTGAATAATATCGACAATGTTTTAGTAAAA
    GTGTTGGATCCACTATTTGCAGGATTTACGGTAGAACATGATTACGATATTACTTCTAAA
    ACGATTCAACCAAAACCAGGAGAAAGTGTTGGACGTTTAGTTAATGTCGATTGTAAAGAT
    ACAGTACTGGAGTATTCAGAATTAGATCCAGAAGTTGCAAATCAATTTAACAATGCTAAT
    ATCGGTATTCATGCATTTAAACTTGGTTTTATTTTAAACGCTGTAAATCGTGAGTTACCT
    TATCATTTAGCTATTAAGAATTTGAAACAATTAGATGAGAACTTTGGGGTTATTGAACAA
    CCAACTTTAAAATTCGAATTGTTCTATTTTGACATATTTACTTATGGAACTAGTTTTGTC
    ACGTTACAAGTTCCTAGAGAAGAAGAATTTTCACCTCTTAAAAATAAAGAAGGTAAAGAT
    AGTGTTGCAACTGCAACAGAAGATTTACGTAGAATGGGTTTAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1974 [new locus tag: SA_RS11370 ]
  • symbol: SA1974
  • description: hypothetical protein
  • length: 395
  • theoretical pI: 4.60106
  • theoretical MW: 44865.4
  • GRAVY: -0.39038

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.-?  ExPASy
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 17)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 13)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 11.2)
  • TheSEED  :
    • N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
  • PFAM:
    GT-A (CL0110) UDPGP; UTP--glucose-1-phosphate uridylyltransferase (PF01704; HMM-score: 142.2)
    and 1 more
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001485
    • TAT(Tat/SPI): 0.000152
    • LIPO(Sec/SPII): 0.000474
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAKLYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGGQGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYFEAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYLEEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNVDCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDENFGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRMGLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1517(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    SA1966hypothetical protein  [1] (data from MRSA252)
    SA1967hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, LexA (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    LexA(TF)important in SOS response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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