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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1966 [new locus tag: SA_RS11280 ]
  • pan locus tag?: SAUPAN005507000
  • symbol: SA1966
  • pan gene symbol?: cdaR
  • synonym: ybbR
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1966 [new locus tag: SA_RS11280 ]
  • symbol: SA1966
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2227250..2228182
  • length: 933
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCTAGAAAGTAAATGGGGCTTGAGATTTATTGCCTTTCTTTTGGCATTGTTTTTCTTT
    TTATCTGTTAATAATGTTTTTGGAAATATCTTTAACACTGGTAATCTTGGTCAAAAGTCT
    AGTAAAACGATTCAAGATGTACCCGTTGAAATTCTTTATAACACTAAAGATTTGCATTTA
    ACAAAAGCGCCTGAAACAGTTAATGTGACTATTTCAGGACCACAATCAAAGATAATAAAA
    ATTGAAAATCCAGAAGATTTAAGAGTAGTGATTGATTTATCAAATGCTAAAGCTGGAAAA
    TATCAAGAGAAGTATCAAGTTAAAGGGTTAGCTGATGACATTCATTATTCTGTAAAACCT
    AAATTAGCAAATATTACGCTTGAAAACAAAGTAACTAAAAAGATGACAGTTCAACCTGAT
    GTAAGTCAGAGTGATATTGATCCACTTTATAAAATTACAAAGCAAGAAGTTTCACCACAA
    ACAGTTAAAGTAACAGGTGGAGAAGAACAATTGAATGATATCGCTTATTTAAAAGCCACT
    TTTAAAACTAATAAAAAGATTAATGGTGACACAAAAGATGTCGCAGAAGTAACGGCTTTT
    GATAAAAAACTGAATAAATTAAATGTATCGATTCAACCTAATGAAGTGAATTTACAAGTT
    AAAGTAGAGCCTTTTAGCAAAAAGGTTAAAGTAAATGTTAAACAGAAAGGTAGTTTAGCA
    GATGATAAAGAGTTAAGTTCGATTGATTTAGAAGATAAAGAAATTGAAATCTTCGGTAGT
    CGAGATGACTTACAAAATATAAGCGAAGTTGATGCAGAAGTAGATTTAGATGGTATTTCA
    GAATCAACTGAAAAGACTGTAAAAATCAATTTACCAGAACATGTCACTAAAGCACAACCA
    AGTGAAACGAAGGCTTATATAAATGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1966 [new locus tag: SA_RS11280 ]
  • symbol: SA1966
  • description: hypothetical protein
  • length: 310
  • theoretical pI: 6.32995
  • theoretical MW: 34624.2
  • GRAVY: -0.443871

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • uncharacterized secreted protein, YBBR Bacillus subtilis homolog
  • PFAM:
    no clan defined YbbR; YbbR-like protein (PF07949; HMM-score: 97.2)
    and 2 more
    E-set (CL0159) DUF3244; Domain of unknown function (DUF3244) (PF11589; HMM-score: 12.2)
    ATP_synthase (CL0255) ATP-synt_8; ATP synthase protein 8 (PF00895; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.846006
    • TAT(Tat/SPI): 0.002594
    • LIPO(Sec/SPII): 0.02281
    • Cleavage Site: CS pos: 28-29. VFG-NI. Pr: 0.7728
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTIQDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQEKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQTVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNLQVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDLDGISESTEKTVKINLPEHVTKAQPSETKAYINVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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