PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1207 [new locus tag: SA_RS06860 ]
• pan locus tag^?: SAUPAN003770000
• symbol: femB
• pan gene symbol^?: femB
• synonym:
• product: FemB protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1207 [new locus tag: SA_RS06860 ]
• symbol: femB
• product: FemB protein
• replicon: chromosome
• strand: +
• coordinates: 1380485..1381744
• length: 1260
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124046 NCBI
• RefSeq: NP_374488 NCBI
• BioCyc: see SA_RS06860
• MicrobesOnline: 103514 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGAAATTTACAGAGTTAACTGTTACCGAATTTGACAACTTTGTACAAAATCCATCATTG
  GAAAGTCATTATTTCCAAGTAAAAGAAAATATAGTTACCCGTGAGAATGATGGCTTTGAA
  GTAGTTTTATTAGGTATTAAAGACGACAATAACAAAGTAATTGCAGCAAGCCTTTTCTCT
  AAAATTCCTACTATGGGAAGTTATGTTTACTATTCGAATCGTGGTCCAGTAATGGATTTT
  TCAGATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACAT
  CAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCATCTATATGATAAAGATATC
  GTGCCATTTGAAGGTCGCGAGAAAAATGATGCCTTAGTAAACTTGTTTAAATCACATGGT
  TACGAGCATCATGGCTTTACAACTGAGTATGATACATCGAGCCAAGTACGATGGATGGGC
  GTATTAAACCTTGAAGGTAAAACACCCGAAACATTGAAAAAGACATTTGATAGTCAACGT
  AAACGTAATATTAATAAAGCGATAAACTATGGTGTTAAAGTCAGATTCCTTGAACGTGAT
  GAGTTCAATCTTTTCTTAGATTTATATCGTGAAACTGAAGAGCGTGCTGGATTTGTGTCA
  AAAACAGATGATTATTTTTATAACTTTATTGACACATATGGAGATAAAGTATTAGTACCA
  TTAGCATATATTGACCTTGATGAATATGTGTTAAAGTTGCAACAGGAATTGAATGACAAA
  GAAAATCGTCGTGATCAAATGATGGCGAAAGAAAACAAATCAGATAAACAAATGAAGAAA
  ATTGCAGAATTAGATAAGCAAATTGATCATGATCAGCATGAATTATTGAATGCAAGTGAA
  TTGAGCAAAACGGACGGCCCAATTCTAAACCTTGCTTCTGGCGTTTATTTTGCAAATGCA
  TATGAAGTGAATTATTTCTCTGGTGGTTCATCAGAAAAATATAATCAATTTATGGGACCA
  TACATGATGCATTGGTTTATGATTAACTATTGCTTCGATAATGGCTATGATCGTTATAAT
  TTCTATGGTTTATCAGGTGATTTTACGGAAAACAGTGAAGATTATGGCGTATACCGCTTT
  AAACGTGGATTTAATGTACAAATCGAAGAATTAATAGGGGATTTCTATAAACCAATTCAT
  AAAGTGAAATATTGGTTGTTCACAACATTGGATAAATTACGTAAAAAATTAAAGAAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  

⊟Protein

⊟General

• locus tag: SA1207 [new locus tag: SA_RS06860 ]
• symbol: FemB
• description: FemB protein
• length: 419
• theoretical pI: 5.37676
• theoretical MW: 49675.6
• GRAVY: -0.649881

⊟Function

• reaction:
  EC 2.3.2.18?  ExPASy
  N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N⁶-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N⁶-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA
• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major capsid protein, HK97 family (TIGR01554; HMM-score: 12.1)
• TheSEED  :
  • FemB, factor involved in methicillin resistance
  • tRNA-dependent lipid II-GlyGlyGly glycyltransferase (EC 2.3.2.18)
  • tRNA-dependent lipid II-GlyGlyGlyGly glycyltransferase (EC 2.3.2.18)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory tRNA-dependent amino acid transfers  tRNA-dependent lipid II-GlyGlyGly--glycine ligase
  and 1 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory tRNA-dependent amino acid transfers  tRNA-dependent lipid II-GlyGlyGlyGly--glycine ligase
• PFAM:
  Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 471.9)
  and 2 more
  Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 18.7)
  no clan defined DUF1497; Protein of unknown function (DUF1497) (PF07384; HMM-score: 14.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004602
  • TAT(Tat/SPI): 0.000131
  • LIPO(Sec/SPII): 0.000248
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926955 NCBI
• RefSeq: NP_374488 NCBI
• UniProt: P0A0A7 UniProt

⊟Protein sequence

• MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMGVLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIHKVKYWLFTTLDKLRKKLKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: femA > femB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications