From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • pan locus tag?: SAUPAN003770000
  • symbol: femB
  • pan gene symbol?: femB
  • synonym:
  • product: methicillin resistance expression factor FemB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • symbol: femB
  • product: methicillin resistance expression factor FemB
  • replicon: chromosome
  • strand: +
  • coordinates: 1418312..1419571
  • length: 1260
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAATTTACAGAGTTAACTGTTACCGAATTTGACAACTTTGTACAAAATCCATCATTG
    GAAAGTCATTATTTCCAAGTAAAAGAAAATATAGTTACCCGTGAGAATGATGGCTTTGAA
    GTAGTTTTATTAGGTATTAAAGACGACAATAACAAAGTAATTGCAGCAAGCCTTTTCTCT
    AAAATTCCTACTATGGGAAGTTATGTTTACTATTCGAATCGTGGTCCAGTAATGGATTTT
    TCAGATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACAT
    CAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCATCTATATGATAAAGATATC
    GTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGT
    TACGAGCATCATGGCTTTACAACTGAGTATGATACATCGAGCCAAGTACGATGGATGGGC
    GTATTAAACCTTGAAGGTAAAACACCCGAAACATTGAAAAAGACATTTGATAGTCAACGT
    AAACGTAATATTAATAAAGCGATAAACTATGGTGTTAAAGTCAGATTCCTTGAACGTGAT
    GAGTTCAATCTTTTCTTAGATTTATATCGTGAAACTGAAGAGCGTGCTGGATTTGTATCA
    AAAACAGATGATTATTTTTATAACTTTATTGACACATATGGAGATAAAGTATTAGTACCA
    TTAGCATATATTGACCTTGATGAATATGTGTTAAAGTTGCAACAGGAATTGAATGACAAA
    GAAAATCGTCGTGATCAAATGATGGCGAAAGAAAACAAATCAGATAAGCAAATGAAGAAA
    ATTGCAGAATTAGATAAGCAAATTGATCATGATCAGCATGAATTATTGAATGCAAGTGAA
    TTGAGCAAAACGGACGGCCCAATTCTAAACCTTGCTTCTGGCGTTTATTTTGCAAATGCA
    TATGAAGTGAATTATTTCTCTGGTGGTTCATCAGAAAAATATAATCAATTTATGGGACCA
    TACATGATGCATTGGTTTATGATTAACTATTGCTTCGATAATGGCTATGATCGTTATAAT
    TTCTATGGTTTATCAGGTGATTTTACGGAAAACAGTGAAGATTATGGCGTATACCGCTTT
    AAACGTGGATTTAATGTACAAATCGAAGAATTAATAGGGGATTTCTATAAACCAATTCAT
    AAAGTGAAATATTGGTTGTTCACAACATTGGATAAATTACGTAAAAAATTAAAGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1287 [new locus tag: NWMN_RS07250 ]
  • symbol: FemB
  • description: methicillin resistance expression factor FemB
  • length: 419
  • theoretical pI: 5.37676
  • theoretical MW: 49675.6
  • GRAVY: -0.649881

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major capsid protein, HK97 family (TIGR01554; HMM-score: 12.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 471.9)
    and 2 more
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 18.7)
    no clan defined DUF1497; Protein of unknown function (DUF1497) (PF07384; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004602
    • TAT(Tat/SPI): 0.000131
    • LIPO(Sec/SPII): 0.000248
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMGVLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIHKVKYWLFTTLDKLRKKLKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_1287&oldid=64220"