From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1205 [new locus tag: SA_RS06850 ]
  • pan locus tag?: SAUPAN003768000
  • symbol: trpA
  • pan gene symbol?: trpA
  • synonym:
  • product: tryptophan synthase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1205 [new locus tag: SA_RS06850 ]
  • symbol: trpA
  • product: tryptophan synthase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1378154..1378882
  • length: 729
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACTAAATTATTTATACCTTATATTATGGGCAATAAAGATTTGATTGAAAATGCAACA
    TTGTTGAGTGAAAATGGTGCAGATATAATTGAAATTGGTGTACCTTTCTCTGATCCGGTT
    GCTGATGGTCCAGTTATCATGGAAGCAGGTCAACAAGCGATTAAACAAGGCATCACGATA
    GATTATATTTTCAATCAATTAGAAAAACATGGTGATCAAATTAAGTGTAACTATGTATTA
    ATGACGTATTATAATATTATTTGTCATTATGGAGAACAAGCGTTTTTTGAAAAATGTCGA
    GATACTGGTGTCTACGGCTTAATTATTCCTGATTTACCATATGAATTATCGCAGCGTTTA
    AAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGAT
    GACAAACGTATAAAAGATATCGTATCTCATGCGGAAGGCTTTATTTATACTGTGACGATG
    AATGCGACAACAGGACAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCA
    ATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACAT
    GTTGCAGATATAAAAGAGGTTGCAGATGGCATCGTCATTGGTAGCGAAATCGTTAAGCGA
    TTTAAATCTAACACGCGTGAGGAAATCATTAGATATTTACAATCTATCCAACAAACATTG
    AATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    729

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1205 [new locus tag: SA_RS06850 ]
  • symbol: TrpA
  • description: tryptophan synthase subunit alpha
  • length: 242
  • theoretical pI: 6.24515
  • theoretical MW: 27145.1
  • GRAVY: -0.0909091

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.20?  ExPASy
    Tryptophan synthase L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 209.6)
    and 4 more
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 17.4)
    Genetic information processing Protein fate Protein folding and stabilization membrane complex biogenesis protein, BtpA family (TIGR00259; HMM-score: 16.8)
    Cellular processes Cellular processes Sporulation and germination stage II sporulation protein M (TIGR02831; HMM-score: 13.6)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.5)
  • TheSEED  :
    • Tryptophan synthase alpha chain (EC 4.2.1.20)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Tryptophan synthase alpha chain (EC 4.2.1.20)
  • PFAM:
    TIM_barrel (CL0036) Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 248.7)
    and 4 more
    BtpA; BtpA family (PF03437; HMM-score: 18.3)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 16.2)
    DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 15.8)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004206
    • TAT(Tat/SPI): 0.000134
    • LIPO(Sec/SPII): 0.000496
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMNATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTLNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: T-box(Trp) (transcription antitermination) regulon, CodY (repression) regulon
    T-box(Trp)(RNA)important in Amino acid metabolism; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1205&oldid=94378"