PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1285 [new locus tag: NWMN_RS07240 ]
• pan locus tag^?: SAUPAN003768000
• symbol: trpA
• pan gene symbol^?: trpA
• synonym:
• product: tryptophan synthase subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1285 [new locus tag: NWMN_RS07240 ]
• symbol: trpA
• product: tryptophan synthase subunit alpha
• replicon: chromosome
• strand: +
• coordinates: 1415981..1416709
• length: 729
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332562 NCBI
• RefSeq: YP_001332319 NCBI
• BioCyc:
• MicrobesOnline: 3706837 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGACTAAATTATTTATACCTTATATTATGGGCAATAAAGATTTGATTGAAAATGCAACA
  TTGTTGAGTGAAAATGGTGCAGATATAATTGAAATTGGAGTACCTTTCTCTGATCCGGTT
  GCTGATGGTCCAGTTATCATGGAAGCAGGTCAACAAGCGATTAAACAAGGCATCACGATA
  GATTATATTTTCAATCAATTAGAAAAACATGGTGATCAAATTAAGTGTAACTATGTATTA
  ATGACGTATTATAATATTATTTGTCATTATGGAGAACAAGCGTTTTTTGAAAAATGTCGA
  GATACTGGTGTCTACGGCTTAATTATTCCTGATTTACCATATGAATTATCGCAGCGTTTA
  AAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGAT
  GACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATG
  AATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCA
  ATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACAT
  GTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGA
  TTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCCAACAAACATTG
  AATAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  729
  

⊟Protein

⊟General

• locus tag: NWMN_1285 [new locus tag: NWMN_RS07240 ]
• symbol: TrpA
• description: tryptophan synthase subunit alpha
• length: 242
• theoretical pI: 6.24515
• theoretical MW: 27117.1
• GRAVY: -0.0884297

⊟Function

• reaction:
  EC 4.2.1.20?  ExPASy
  Tryptophan synthase L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H₂O
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 210.2)
  and 4 more
  Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 17.8)
  Genetic information processing Protein fate Protein folding and stabilization membrane complex biogenesis protein, BtpA family (TIGR00259; HMM-score: 16)
  geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.5)
  Cellular processes Cellular processes Sporulation and germination stage II sporulation protein M (TIGR02831; HMM-score: 13.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 249)
  and 4 more
  BtpA; BtpA family (PF03437; HMM-score: 18.2)
  His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 16.3)
  DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 16.2)
  IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004206
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000496
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221497 NCBI
• RefSeq: YP_001332319 NCBI
• UniProt: A6QGS5 UniProt

⊟Protein sequence

• MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMNATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIKYLQSIQQTLNN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: trpE > trpG > trpD > trpC > trpF > trpB > trpA

⊟Regulation

• regulators: T-box(Trp) (transcription antitermination) regulon, CodY (repression) regulon

  T-box(Trp)	(RNA)	important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications