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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1045 [new locus tag: SA_RS05940 ]
  • pan locus tag?: SAUPAN003482000
  • symbol: pyrAA
  • pan gene symbol?: carA
  • synonym:
  • product: carbamoyl phosphate synthase small subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1045 [new locus tag: SA_RS05940 ]
  • symbol: pyrAA
  • product: carbamoyl phosphate synthase small subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1182038..1183138
  • length: 1101
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCAAAGCAAACGTTATCTAGTGTTAGAAGACGGTTCTTTTTACGAAGGCTACCGTTTA
    GGATCTGATAACTTAACTGTAGGAGAAATTGTATTTAATACAGCTATGACAGGTTATCAA
    GAAACTATTTCAGATCCATCATATACAGGTCAGATCATTACTTTTACGTATCCATTAATC
    GGTAATTATGGTATCAATAGAGACGATTTTGAATCATTAGTACCTACATTAAACGGTATT
    GTAGTGAAAGAAGCGAGTGCGCATCCAAGTAATTTTAGACAACAAAAGACACTTCATGAC
    GTTTTAGAATTGCATCAAATTCCAGGGATTGCAGGTGTTGATACAAGAAGTATTACGCGT
    AAAATTCGACAACACGGTGTGTTAAAAGCTGGTTTTACTGATCGAAAAGAAGATATTGAT
    CAACTTGTCAAACATTTACAACAAGTAGAATTACCTAAAAACGAAGTAGAAATCGTTTCG
    ACTAAAACACCGTATGTTTCGACAGGTAAGGATCTAAGTGTCGTACTTGTAGACTTTGGT
    AAGAAGCAAAATATTGTTCGAGAATTAAACGTCAGAGGTTGTAACGTCACAGTTGTACCA
    TATACAACTACTGCCGAAGAAATTTTAGCAATGGCTCCAGATGGCGTTATGCTATCAAAC
    GGACCAGGTAATCCTGAAGTTGTAGAATGTGCGATTCCAATGATTCAAGGAATTTTAGGG
    AAAATTCCGTTCTTTGGTATCTGTCTAGGACATCAACTTTTTGCATTATCTCAAGGAGCA
    AGCTCATTTAAAATGAAGTTTGGTCATCGTGGTGCGAACCATCCAGTTAAAAATTTAGAG
    ACTGGAAAAGTTGATATTACGAGTCAAAACCATGGATATGCAATAGATATAGATTCGTTA
    AAAAGTACTGATTTAGAAGTTACTCATCTTGCATTAAATGATGGTACTGTAGAAGGTTTA
    AAACATAAAACATTACCAGCATTTTCTGTTCAATACCATCCTGAAGCAAATCCAGGACCG
    TCAGATTCAAACTATCTATTTGATGATTTTGTAGCAATGATGACTAATTTTAAGGAAAAG
    GAGCGTCATATCAATGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1101

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1045 [new locus tag: SA_RS05940 ]
  • symbol: PyrAA
  • description: carbamoyl phosphate synthase small subunit
  • length: 366
  • theoretical pI: 6.18896
  • theoretical MW: 40393.6
  • GRAVY: -0.25847

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.5.5?  ExPASy
    Carbamoyl-phosphate synthase (glutamine-hydrolyzing) 2 ATP + L-glutamine + HCO3(-) + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 484.5)
    and 5 more
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 62.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 59.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 50.1)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 48.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 20)
  • TheSEED  :
    • Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)
    Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)
  • PFAM:
    Leu-IlvD (CL0364) CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain (PF00988; HMM-score: 167.7)
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 162.1)
    and 1 more
    Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 30.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016429
    • TAT(Tat/SPI): 0.000433
    • LIPO(Sec/SPII): 0.00096
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: pyrR leader (transcription antitermination) regulon
    pyrR leader(RNA)important in Pyrimidine metabolism; RegPrecise binding of the PyrR antitermination protein

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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