PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1041 [new locus tag: SA_RS05920 ]
• pan locus tag^?: SAUPAN003478000
• symbol: pyrR
• pan gene symbol^?: pyrR
• synonym:
• product: bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1041 [new locus tag: SA_RS05920 ]
• symbol: pyrR
• product: bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase
• replicon: chromosome
• strand: +
• coordinates: 1177783..1178310
• length: 528
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123872 NCBI
• RefSeq: NP_374314 NCBI
• BioCyc: see SA_RS05920
• MicrobesOnline: 103340 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGTCTGAACGTATTATAATGGATGATGCCGCAATACAACGTACAGTGACGAGAATCGCT
  CACGAAATTTTGGAGTATAACAAAGGTACTGATAATTTAATTCTTTTAGGTATCAAAACA
  AGAGGTGAATATTTAGCGAATCGTATACAAGATAAAATTCATCAAATTGAGCAACAACGT
  ATACCTACTGGAACAATTGATATTACATACTTTAGAGATGATATAGAGCACATGTCATCA
  CTTACGACAAAAGACGCAATAGACATCGACACAGATATTACAGATAAAGTAGTCATCATT
  ATTGATGATGTGCTGTATACTGGTCGAACGGTTCGTGCTTCACTTGATGCTATTTTGCTA
  AATGCTAGACCTATTAAAATTGGTTTAGCTGCTTTGGTTGATCGAGGACATCGTGAGTTA
  CCAATTCGAGCAGATTTTGTTGGTAAAAATATACCTACTTCTAAAGAGGAAACGGTAAGT
  GTCTATTTAGAAGAAATGGATCAAAGAAATGCAGTTATAATTAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  528
  

⊟Protein

⊟General

• locus tag: SA1041 [new locus tag: SA_RS05920 ]
• symbol: PyrR
• description: bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase
• length: 175
• theoretical pI: 5.08873
• theoretical MW: 19854.6
• GRAVY: -0.196

⊟Function

• reaction:
  EC 2.4.2.9?  ExPASy
  Uracil phosphoribosyltransferase UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 44.3)
  and 5 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 17.1)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 16.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 15.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 13.9)
  Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 11.5)
• TheSEED  :
  • Pyrimidine operon regulatory protein PyrR
  Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Pyrimidine operon regulatory protein PyrR
  and 2 more
  Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Uracil phosphoribosyltransferase (EC 2.4.2.9)
  Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Uracil phosphoribosyltransferase (EC 2.4.2.9)
• PFAM:
  PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 68.4)
  and 2 more
  PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 13.6)
  PRTase_3; PRTase ComF-like (PF15610; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002036
  • TAT(Tat/SPI): 0.000168
  • LIPO(Sec/SPII): 0.000277
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926781 NCBI
• RefSeq: NP_374314 NCBI
• UniProt: P65944 UniProt

⊟Protein sequence

• MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEYLANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIIDDVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVSVYLEEMDQRNAVIIK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pyrR > pyrP > pyrB > pyrC > pyrAA > carB > pyrF > pyrE > SA1049

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications