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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0831 [new locus tag: SA_RS04720 ]
  • pan locus tag?: SAUPAN003107000
  • symbol: cdr
  • pan gene symbol?: cdr
  • synonym:
  • product: coenzyme A disulfide reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0831 [new locus tag: SA_RS04720 ]
  • symbol: cdr
  • product: coenzyme A disulfide reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 941465..942781
  • length: 1317
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCCAAAATAGTCGTAGTCGGAGCAGTCGCTGGCGGTGCAACATGTGCCAGCCAAATT
    CGACGTTTAGATAAAGAAAGTGACATTATTATTTTTGAAAAAGATCGTGATATGAGCTTT
    GCTAATTGTGCATTGCCTTATGTCATTGGCGAAGTTGTTGAAGATAGAAAATATGCTTTA
    GCGTATACACCTGAAAAATTTTATGATAGAAAGCAAATTACAGTAAAAACTTATCATGAA
    GTTATTGCAATCAATGATGAAAGACAAACTGTAACTGTATTAAATAGAAAGACCAACGAA
    CAATTTGAAGAATCTTACGATAAACTCATTTTAAGCCCTGGTGCAAGTGCAAATAGCCTT
    GGCTTTGAAAGTGATATTACATTTACACTTAGAAATTTAGAAGACACTGATGCTATCGAT
    CAATTCATCAAAGCAAATCAAGTTGATAAAGTATTGGTTATAGGTGCAGGGTATGTTTCA
    TTAGAAGTTCTTGAAAATCTTTACGAACGTGGTTTACACCCTACTTTAATTCATCGATCT
    GATAAGATAAATAAATTAATGGATGCTGACATGAATCAACCTATACTTGATGAATTAGAT
    AAGCGGGAGATTCCATACCGTTTAAATGAGGAAATTGATGCTATCAATGGAAATGAAATT
    ACATTTAAATCAGGAAAAGTTGAACATTACGATATGATTATTGAAGGTGTCGGTACTCAC
    CCCAATTCAAAATTTATCGAAAGTTCAAATATCAAACTTGATCGAAAAGGTTTCATACCG
    GTAAACGATAAATTTGAAACAAATGTTCCAAACATTTATGCAATAGGCGATATTGCAACA
    TCACATTATCGACATGTCGATCTACCGGCTAGTGTTCCTTTAGCTTGGGGCGCTCACCGT
    GCAGCAAGTATTGTTGCCGAACAAATTGCTGGAAATGACACTATTGAATTCAAAGGCTTC
    TTAGGCAACAATATTGTGAAGTTCTTTGATTATACATTTGCGAGTGTCGGCGTTAAACCA
    AATGAACTAAAGCAATTTGACTATAAAATGGTGGAAGTCACTCAAGGTGCACACGCGAAT
    TATTACCCAGGAAATTCCCCTTTACACTTAAGGGTATATTATGACACTTCAAACCGTCAG
    ATTTTAAGAGCAGCTGCAGTAGGAAAAGAAGGTGCAGATAAACGTATTGATGTACTATCG
    ATGGCAATGATGAACCAGCTAACTGTAGATGAGTTAACTGAGTTTGAAGTGGCTTATGCA
    CCACCATATAGCCACCCTAAAGATTTAATCAATATGATTGGTTACAAAGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0831 [new locus tag: SA_RS04720 ]
  • symbol: Cdr
  • description: coenzyme A disulfide reductase
  • length: 438
  • theoretical pI: 5.13828
  • theoretical MW: 49290.6
  • GRAVY: -0.309132

⊟Function[edit | edit source]

  • reaction:
    EC 1.8.1.14?  ExPASy
    CoA-disulfide reductase 2 CoA + NADP+ = CoA-disulfide + NADPH
  • TIGRFAM:
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 688.8)
    and 20 more
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 105.3)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 104.4)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 71.8)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 64.1)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 63.9)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 58.1)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 57.2)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 52.3)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 52.1)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 47.1)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 46.2)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 41.9)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 30.5)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 30.3)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 30.3)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 25.6)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 15.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 14.4)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 12.3)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 11.7)
  • TheSEED  :
    • CoA-disulfide reductase (EC 1.8.1.14)
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 172.9)
    and 11 more
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 58.7)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 52.4)
    Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 40.4)
    NADP_Rossmann (CL0063) K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 18.8)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17.1)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 15.8)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.6)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 14.6)
    RNase_H (CL0219) DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 13.1)
    NADP_Rossmann (CL0063) Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 12.7)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 15 aa
    • SP(Sec/SPI): 0.029443
    • TAT(Tat/SPI): 0.00153
    • LIPO(Sec/SPII): 0.639082
    • Cleavage Site: CS pos: 15-16. GAT-CA. Pr: 0.6201
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCALPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQFEESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVIGAGYVSLEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGNEITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIGDIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFASVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGADKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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