PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0342 [new locus tag: SA_RS01960 ]
• pan locus tag^?: SAUPAN001898000
• symbol: SA0342
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0342 [new locus tag: SA_RS01960 ]
• symbol: SA0342
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 399116..400297
• length: 1182
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123121 NCBI
• RefSeq: NP_373588 NCBI
• BioCyc: see SA_RS01960
• MicrobesOnline: 102614 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGACGAGAGTCGTATTAGCAGCAGCATACAGGACACCTATTGGCGTTTTTGGAGGTGCG
  TTTAAAGACGTGCCAGCCTATGATTTAGGTGCGACTTTAATAGAACATATTATTAAAGAG
  ACGGGTTTGAATCCAAGTGAGATTGATGAAGTTATCATCGGTAACGTACTACAAGCAGGA
  CAAGGACAAAATCCAGCACGAATTGCTGCTATGAAAGGTGGCTTGCCAGAAACAGTACCT
  GCATTTACAGTGAATAAAGTATGTGGTTCTGGGTTAAAGTCGATTCAATTAGCATATCAA
  TCTATTGTGACTGGTGAAAATGACATCGTGCTAGCTGGCGGTATGGAGAATATGTCTCAG
  TCACCAATGCTTGTCAACAACAGTCGCTTCGGTTTTAAAATGGGACATCAATCAATGGTT
  GATAGCATGGTATATGATGGTTTAACAGATGTATTTAATCAATATCATATGGGTATTACT
  GCTGAAAATTTAGTAGAGCAATATGGTATTTCAAGAGAAGAACAAGATACATTTGCTGTA
  AACTCACAACATAAAGCAGTACGTGCACAGCAAAATGGTGAATTTGATAGTGAAATAGTT
  CCAGTATCGATTCCTCAACGTAAAGGTGAACCAATCTTAGTCACTAAGGATGAAGGTGTA
  CGTGAAAATGTATCAGTCGAAAAATTAAGTCGCTTAAGACCAGCTTTCAAAAAAGACGGG
  ACAGTTACAGCAGGTAATGCATCAGGAATCAATGATGGTGCTGCGATGATGTTAGTCATG
  TCAGAAGACAAAGCTAAAGAATTAAATATCGAACCATTGGCAGTGCTTGATGGCTTTGGA
  AGTCATGGCGTAGATCCTTCTATTATGGGTATTGCACCAGTTGGCGCTGTAGAAAAGGCT
  TTGAAACGTAGTAAAAAAGAATTAAGCGATATTGATGTATTTGAATTAAATGAAGCATTT
  GCAGCACAATTATTAGCTGTTGATCGTGAATTAAAATTACCTCCTGAAAAGGTGAATGTT
  AAAGGTGGCGCTATTGCATTAGGACACCCTATTGGTGCATCTGGTGCTAGAGTTTTAGTG
  ACATTATTGCATCAACTGAATGATGAAGTTGAAACTGGTTTAACATCATTGTGTATTGGT
  GGCGGTCAAGCTATCGCTGCAGTTGTATCAAAGTATAAATAA
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  1140
  1182
  

⊟Protein

⊟General

• locus tag: SA0342 [new locus tag: SA_RS01960 ]
• symbol: SA0342
• description: hypothetical protein
• length: 393
• theoretical pI: 5.03642
• theoretical MW: 41752.5
• GRAVY: -0.0274809

⊟Function

• reaction:
  EC 2.3.1.9?  ExPASy
  Acetyl-CoA C-acetyltransferase 2 acetyl-CoA = CoA + acetoacetyl-CoA
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 498.3)
  and 5 more
  3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 386.8)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 329.9)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 188.3)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 27)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 16.9)
• TheSEED  :
  • 3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  • Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  and 9 more
  Carbohydrates Fermentation Acetyl-CoA fermentation to Butyrate  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Carbohydrates Fermentation Butanol Biosynthesis  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
• PFAM:
  Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 362.3)
  and 4 more
  Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 151.6)
  ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 28.2)
  ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 14.1)
  FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02427
  • TAT(Tat/SPI): 0.001086
  • LIPO(Sec/SPII): 0.0046
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926055 NCBI
• RefSeq: NP_373588 NCBI
• UniProt: Q7A7L2 UniProt

⊟Protein sequence

• MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLNPSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSIVTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGITAENLVEQYGISREEQDTFAVNSQHKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDEGVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVLDGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQLLAVDRELKLPPEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKYK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)