PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000308
• pan locus tag^?: SAUPAN001898000
• symbol: JSNZ_000308
• pan gene symbol^?: —
• synonym:
• product: acetyl-CoA C-acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000308
• symbol: JSNZ_000308
• product: acetyl-CoA C-acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 343952..345133
• length: 1182
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGACGAGAGTCGTATTAGCAGCAGCATACAGGACACCTATTGGCGTTTTTGGAGGTGCG
  TTTAAAGACGTGCCAGCCTATGATTTAGGTGCGACTTTAATAGAACATATTATTAAAGAG
  ACGGGTTTGAATCCAAGTGAGATTGATGAAGTTATCATCGGTAACGTACTACAAGCAGGA
  CAAGGACAAAATCCAGCACGAATTGCTGCTATGAAAGGTGGCTTGCCAGAAACAGTACTT
  GCATTTACAGTGAATAAAGTATGTGGTTCTGGGTTAAAGTCGATTCAATTAGCATATCAA
  TCTATTGTGACTGGTGAAAATGACATCGTGCTAGCTGGCGGTATGGAGAATATGTCTCAG
  TCACCAATGCTTGTCAACAACAGTCGCTTCGGTTTTAAAATGGGACATCAATCAATGGTT
  GATAGCATGGTATATGATGGTTTAACAGATGTATTTAATCAATATCATATGGGTATTACT
  GCTGAAAATTTAGTGGAGCAATATGGTATTTCAAGAGAAGAACAAGATACATTTGCTGTA
  AACTCACAACAAAAAGCAGTACGTGCACAGCAAAATGGTGAATTTGATAGTGAAATAGTT
  CCAGTATCGATTCCTCAACGTAAAGGTGAACCAATCTTAGTCACTAAGGATGAAGGTGTA
  CGTGAAAATGTATCAGTCGAAAAATTAAGTCGCTTAAGACCAGCTTTCAAAAAAGACGGT
  ACAGTTACAGCAGGTAATGCATCAGGAATCAATGATGGTGCTGCGATGATGTTAGTCATG
  TCAGAAGACAAAGCTAAAGAATTAAATATCGAACCATTGGCAGTGCTTGATGGCTTTGGA
  AGTCATGGTGTAGATCCTTCTATTATGGGTATTGCACCAGTTGGCGCTGTAGAAAAGGCT
  TTGAAACGTAGTAAAAAAGAATTAAGCGATATTGATGTATTTGAATTAAATGAAGCATTT
  GCAGCACAATCATTAGCTGTTGATCGTGAATTAAAATTACCTCCTGAAAAGGTGAATGTT
  AAAGGTGGCGCTATTGCATTAGGACACCCTATTGGTGCATCTGGTGCTAGAGTTTTAGTG
  ACATTATTGCATCAACTGAATGATGAAGTTGAAACTGGTTTGACATCATTGTGTATTGGT
  GGCGGTCAAGCTATCGCTGCAGTTGTATCAAAGTATAAATAA
  60
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  360
  420
  480
  540
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  720
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  1020
  1080
  1140
  1182
  

⊟Protein

⊟General

• locus tag: JSNZ_000308
• symbol: JSNZ_000308
• description: acetyl-CoA C-acetyltransferase
• length: 393
• theoretical pI: 4.94056
• theoretical MW: 41733.5
• GRAVY: -0.0262087

⊟Function

• reaction:
  EC 2.3.1.9?  ExPASy
  Acetyl-CoA C-acetyltransferase 2 acetyl-CoA = CoA + acetoacetyl-CoA
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 496.3)
  and 5 more
  3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 387.2)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 330.1)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 189.7)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 26.3)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 15.9)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 361.4)
  and 4 more
  Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 154.8)
  ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 26.4)
  Thiolase_C_1; Thiolase C-terminal domain-like (PF22691; HMM-score: 17.9)
  FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.97
  • Cytoplasmic Membrane Score: 0.0077
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.022
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02427
  • TAT(Tat/SPI): 0.001086
  • LIPO(Sec/SPII): 0.0046
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLNPSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVLAFTVNKVCGSGLKSIQLAYQSIVTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGITAENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDEGVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVLDGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLPPEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKYK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications