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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14715 [old locus tag: NWMN_2564 ]
  • pan locus tag?: SAUPAN006410000
  • symbol: NWMN_RS14715
  • pan gene symbol?: icaR
  • synonym:
  • product: biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS14715 [old locus tag: NWMN_2564 ]
  • symbol: NWMN_RS14715
  • product: biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR
  • replicon: chromosome
  • strand: -
  • coordinates: 2832043..2832603
  • length: 561
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2832043..2832603) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2564

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGAAGGATAAGATTATTGATAACGCAATAACCTTATTTTCAGAGAAGGGGTATGACGGT
    ACAACACTTGATGATATAGCTAAAAGTGTAAATATAAAGAAAGCGAGTTTATATTACCAT
    TTTGACTCGAAAAAAAGTATTTACGAACAAAGTGTTAAATGTTGTTTTGATTACCTTAAT
    AATATTATTATGATGAATCAAAATAAATCGAACTATTCAATTGATGCTTTATATCAATTC
    TTATTTGAGTTTATTTTCGACATCGAAGAAAGGTATATTAGAATGTACGTTCAATTATCT
    AATACGCCTGAGGAATTTTCTGGAAATATTTACGGACAAATACAAGATTTAAATCAATCA
    TTAAGTAAAGAGATAGCAAAATTCTATGATGAATCAAAGATAAAGATGACAAAAGAAGAC
    TTTCAGAATTTAATATTGCTGTTTCTTGAAAGTTGGTATTTGAAAGCATCCTTTTCGCAA
    AAATTTGGAGCAGTGGAAGAAAGTAAAAGTCAATTCAAAGATGAAGTGTATTCGCTACTA
    AATATATTTTTGAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    561

⊟Protein[edit | edit source]

Protein Data Bank: 3GEU

⊟General[edit | edit source]

  • locus tag: NWMN_RS14715 [old locus tag: NWMN_2564 ]
  • symbol: NWMN_RS14715
  • description: biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR
  • length: 186
  • theoretical pI: 4.91883
  • theoretical MW: 21986.9
  • GRAVY: -0.376344

⊟Function[edit | edit source]

  • TIGRFAM:
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 33.1)
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 32.8)
    and 1 more
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 26.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 59.1)
    and 2 more
    B-block_TFIIIC; B-block binding subunit of TFIIIC (PF04182; HMM-score: 15.8)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 15)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by IcaR, TF important in Intercellular adhesion: see NWMN_2564

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021785
    • TAT(Tat/SPI): 0.000661
    • LIPO(Sec/SPII): 0.003606
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSKKSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNTPEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQKFGAVEESKSQFKDEVYSLLNIFLKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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