From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002655
  • pan locus tag?: SAUPAN006410000
  • symbol: icaR
  • pan gene symbol?: icaR
  • synonym:
  • product: ica operon transcriptional regulator IcaR

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002655
  • symbol: icaR
  • product: ica operon transcriptional regulator IcaR
  • replicon: chromosome
  • strand: -
  • coordinates: 2673948..2674508
  • length: 561
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGAAGGATAAGATTATTGATAACGCAATAACCTTATTTTCAGAGAAGGGGTATGACGGT
    ACAACACTTGATGATATAGCTAAAAGTGTAAATATAAAGAAAGCGAGTTTATATTACCAT
    TTTGACTCGAAAAAAAGTATTTACGAACAAAGTGTTAAATGTTGTTTTGATTACCTTAAT
    AATATTATTATGATGAATCAAAATAAATCGAACTATTCAATTGATGCTTTATATCAATTC
    TTATTTGAGTTTATTTTCGACATCGAAGAAAGGTATATTAGAATGTACGTTCAATTATCT
    AATACGCCTGAGGAATTTTCTGGAAATATTTACGGACAAATACAAGATTTAAATCAATCA
    TTAAGTAAAGAGATAGCAAAATTCTATGATGAATCAAAGATAAAGATGACAAAAGAAGAC
    TTTCAGAATTTAATATTGCTGTTTCTTGAAAGTTGGTATTTGAAAGCATCCTTTTCGCAA
    AAATTTGGAGCAGTGGAAGAAAGTAAAAGTCAATTCAAAGATGAAGTGTATTCGCTACTA
    AATATATTTTTGAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    561

⊟Protein[edit | edit source]

Protein Data Bank: 3GEU

⊟General[edit | edit source]

  • locus tag: JSNZ_002655
  • symbol: IcaR
  • description: ica operon transcriptional regulator IcaR
  • length: 186
  • theoretical pI: 4.91883
  • theoretical MW: 21986.9
  • GRAVY: -0.376344

⊟Function[edit | edit source]

  • TIGRFAM:
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 33.1)
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 32.8)
    and 1 more
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 26.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TetR_C (CL0174) TetR_C_37; Tetracyclin repressor-like, C-terminal domain (PF18665; HMM-score: 90.9)
    and 3 more
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 59.6)
    B-block_TFIIIC; B-block binding subunit of TFIIIC (PF04182; HMM-score: 16)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by IcaR, TF important in Intercellular adhesionsee RegPrecise for N315
    repression
    icaR
    icaA > icaD > icaB > icaC

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7681
    • Cytoplasmic Membrane Score: 0.1896
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.042
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021785
    • TAT(Tat/SPI): 0.000661
    • LIPO(Sec/SPII): 0.003606
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSKKSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNTPEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQKFGAVEESKSQFKDEVYSLLNIFLKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: IcaR (repression) regulon
    IcaR(TF)important in Intercellular adhesion;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002655&oldid=108296"