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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS13195 [old locus tag: NWMN_2294 ]
- pan locus tag?: SAUPAN005925000
- symbol: NWMN_RS13195
- pan gene symbol?: narI
- synonym:
- product: nitrate reductase subunit gamma
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS13195 [old locus tag: NWMN_2294 ]
- symbol: NWMN_RS13195
- product: nitrate reductase subunit gamma
- replicon: chromosome
- strand: -
- coordinates: 2518936..2519613
- length: 678
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGCTTAATCAGTTTTTATGGGTTATATTTCCGTATTTATGTTTAGTTGTCTTTGTCGCA
GGTCATATTGCACGTTATCGTTATGATAAATTTTCTTGGACAGCTAAATCTAGTGAATTA
ATAGAAAGAAAAAGGTTGATGTGGGGAAGTTTATTATTTCACTTAGGTATTATTCCAGTG
TTTTTTGGACACGTAGTTGGATTGTTAATACCGAAACCATGGATGGATGCAGTGGGTGTG
TCTGAGCATTTATATCATATAGGCGCTGTTTATATTGGTAGCATCTTTGGTATAATAACT
TTGATAGGGATGTTTTTATTAACAGCGAGACGTGTTACTACGAAAAGTATTCGAAGATTA
AGTTCAGCGTCGGATATATTTGTAAATTTCTTATTATTACTTATCGTATTTATGGGATGT
TACGCAACATTAGTAACAAATATACAACATCCAGACTTTAACTACAGAACTTCATTATCA
ATTTGGTTTAGACAATTATTTATGTTAAAACCAGATGCGTCATTGATGAGTGGTGTGCCG
ATTGCATTTAAAATGCATATATTGTTAGGTTTCACTATTATGGCTTGTTGGCCATTTACA
CGTTTGGTGCATGTTTGGAGTGTACCTTTGACATATATAAATCGCAGATATATTATTTAC
CGTAAGAATAAAGTGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS13195 [old locus tag: NWMN_2294 ]
- symbol: NWMN_RS13195
- description: nitrate reductase subunit gamma
- length: 225
- theoretical pI: 10.5772
- theoretical MW: 26145.3
- GRAVY: 0.651111
⊟Function[edit | edit source]
- TIGRFAM: Energy metabolism Anaerobic respiratory nitrate reductase, gamma subunit (TIGR00351; EC 1.7.99.4; HMM-score: 238.5)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined Nitrate_red_gam; Nitrate reductase gamma subunit (PF02665; HMM-score: 293.6)and 1 moreUPF0114; Uncharacterized protein family, UPF0114 (PF03350; HMM-score: 13.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.013116
- TAT(Tat/SPI): 0.000294
- LIPO(Sec/SPII): 0.080568
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLSIWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: NreC* see NWMN_2294
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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