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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09155 [old locus tag: NWMN_1630 ]
  • pan locus tag?: SAUPAN004402000
  • symbol: NWMN_RS09155
  • pan gene symbol?: aroA2
  • synonym:
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09155 [old locus tag: NWMN_1630 ]
  • symbol: NWMN_RS09155
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 1814675..1815766
  • length: 1092
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1814675..1815766) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1630

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGTAATAAATTAGAATCATACAGAAGTGAGATTGTATCACTGAATCATCAAATTTTA
    GACTTATTATCTAAACGTGGTGAACTAGCACAAAAAATTGGGGAAGAAAAATTAAAACAA
    GGTACACGTATTTATGATCCACAACGTGAAAAAGAAATGCTTAACGACTTAATCGATAGT
    AACAAAGGACCATTCAACGATAATACTATTAAGCAATTATTTAAAGAAATTTTCAAAGCC
    TCTACAGATTTACAAAAATCTGAAAATGAAAAACATTTATATGTATCACGTAAGTTGAAA
    CCTGAAGATACGATTGTAACATTTGATAATGGGGGCATTATTGGAGACGGCAATAAATCA
    TTTGTATTTGGGCCATGTTCAGTTGAATCATTTGAACAAGTTGAAGCTGTTGCTAAAAAC
    TTACATGCTAAAGGTGAAAAATTTATTCGTGGCGGTGCATTTAAACCACGTACATCACCA
    TATGATTTCCAAGGCCTAGGTGTTGAAGGACTTAAAATACTTAAACAGATTAAAGATAAA
    TATGATTTAAATGTTGTCAGCGAAATCGTAAATCCAAATGATTTTGAAGTGGCTGATGAG
    TATTTAGACGTATTCCAAATTGGTGCACGTAATATGCAAAACTTCGAGTTATTAAAAGAA
    GCTGGCCGTACGAAAAAGCCTATTCTATTAAAACGTGGTTTATCTGCTACAATCGAAGAG
    TTTGTTTATGCAGCTGAATACATTGCTTCACAAGGTAATCAAAACATTATTTTATGTGAA
    CGTGGAATCCGAACTTATGAAAAGGCGACACGTAACACTTTAGATATTTCAGCAGTACCA
    ATTTTAAAACAAGGTACACACTTACCAGTCATGGTAGATGTTACGCATAGTACAGGTCGT
    AAAGATATCATGTTACCAACTGCGAAAGCAGCATTAGCAGTTGGTGCTGATGGAGTTATG
    GCTGAGGTGCATCCAGATCCATCTGTTGCACTTAGTGATGCGGGTCAACAAATGGATTTA
    GATGAATTCCAAGCATTTTATGATGAATTAAAGCCTTTAGCTGATTTATATAACGCTAAA
    AAGTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS09155 [old locus tag: NWMN_1630 ]
  • symbol: NWMN_RS09155
  • description: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • length: 363
  • theoretical pI: 6.02863
  • theoretical MW: 40618
  • GRAVY: -0.44022

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-deoxy-7-phosphoheptulonate synthase (TIGR01361; EC 2.5.1.54; HMM-score: 381.7)
    and 11 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 170.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-8-phosphooctulonate synthase (TIGR01362; EC 2.5.1.55; HMM-score: 121.6)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 64.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01791; EC 5.4.99.5; HMM-score: 53.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01805; EC 5.4.99.5; HMM-score: 45.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01797; EC 5.4.99.5; HMM-score: 36.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01799; EC 5.4.99.5; HMM-score: 36.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01808; EC 5.4.99.5; HMM-score: 35.4)
    pseudaminic acid synthase (TIGR03586; EC 2.5.1.97; HMM-score: 28.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family putative chorismate mutase (TIGR01806; EC 5.4.99.5; HMM-score: 28.3)
    N-acetylneuraminate synthase (TIGR03569; EC 2.5.1.56; HMM-score: 18.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 287.3)
    and 3 more
    no clan defined CM_2; Chorismate mutase type II (PF01817; HMM-score: 74.1)
    TIM_barrel (CL0036) NeuB; NeuB family (PF03102; HMM-score: 32.7)
    no clan defined SPOB_ab; Sporulation initiation phospho-transferase B, C-terminal (PF14682; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005725
    • TAT(Tat/SPI): 0.000718
    • LIPO(Sec/SPII): 0.000643
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRIYDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPEDTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSPYDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELLKEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDISAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDAGQQMDLDEFQAFYDELKPLADLYNAKKLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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