PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1683 [new locus tag: SAUSA300_RS09195 ]
• pan locus tag^?: SAUPAN004402000
• symbol: SAUSA300_1683
• pan gene symbol^?: aroA2
• synonym:
• product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1683 [new locus tag: SAUSA300_RS09195 ]
• symbol: SAUSA300_1683
• product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
• replicon: chromosome
• strand: -
• coordinates: 1854633..1855724
• length: 1092
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915242 NCBI
• RefSeq: YP_494377 NCBI
• BioCyc: see SAUSA300_RS09195
• MicrobesOnline: 1293198 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  1021
  1081
  
  ATGAGTAATAAATTAGAATCATACAGAAGTGAGATTGTATCACTGAATCATCAAATTTTA
  GACTTATTATCTAAACGTGGTGAACTAGCACAAAAAATTGGGGAAGAAAAATTAAAACAA
  GGTACACGTATTTATGATCCACAACGTGAAAAAGAAATGCTTAACGACTTAATCGATAGT
  AACAAAGGACCATTCAACGATAATACTATTAAGCAATTATTTAAAGAAATTTTCAAAGCC
  TCTACAGATTTACAAAAATCTGAAAATGAAAAACATTTATATGTATCACGTAAGTTGAAA
  CCTGAAGATACGATTGTAACATTTGATAATGGGGGCATTATTGGAGACGGCAATAAATCA
  TTTGTATTTGGGCCATGTTCAGTTGAATCATTTGAACAAGTTGAAGCTGTTGCTAAAAAC
  TTACATGCTAAAGGTGAAAAATTTATTCGTGGCGGTGCATTTAAACCACGTACATCACCA
  TATGATTTCCAAGGCCTAGGTGTTGAAGGACTTAAAATACTTAAACAGATTAAAGATAAA
  TATGATTTAAATGTTGTCAGCGAAATCGTAAATCCAAATGATTTTGAAGTGGCTGATGAG
  TATTTAGACGTATTCCAAATTGGTGCACGTAATATGCAAAACTTCGAGTTATTAAAAGAA
  GCTGGCCGTACGAAAAAGCCTATTCTATTAAAACGTGGTTTATCTGCTACAATCGAAGAG
  TTTGTTTATGCAGCTGAATACATTGCTTCACAAGGTAATCAAAACATTATTTTATGTGAA
  CGTGGAATCCGAACTTATGAAAAGGCGACACGTAACACTTTAGATATTTCAGCAGTACCA
  ATTTTAAAACAAGGTACACACTTACCAGTCATGGTAGATGTTACGCATAGTACAGGTCGT
  AAAGATATCATGTTACCAACTGCGAAAGCAGCATTAGCAGTTGGTGCTGATGGAGTTATG
  GCTGAGGTGCATCCAGATCCATCTGTTGCACTTAGTGATGCGGGTCAACAAATGGATTTA
  GATGAATTCCAAGCATTTTATGATGAATTAAAGCCTTTAGCTGATTTATATAACGCTAAA
  AAGTTAAAATAA
  60
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  1092
  

⊟Protein

⊟General

• locus tag: SAUSA300_1683 [new locus tag: SAUSA300_RS09195 ]
• symbol: SAUSA300_1683
• description: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
• length: 363
• theoretical pI: 6.02863
• theoretical MW: 40618
• GRAVY: -0.44022

⊟Function

• reaction:
  EC 5.4.99.5?  ExPASy
  Chorismate mutase Chorismate = prephenate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aromatic amino acid family 3-deoxy-7-phosphoheptulonate synthase (TIGR01361; EC 2.5.1.54; HMM-score: 381.7)
  and 11 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 170.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-8-phosphooctulonate synthase (TIGR01362; EC 2.5.1.55; HMM-score: 121.6)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 64.4)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01791; EC 5.4.99.5; HMM-score: 53.5)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01805; EC 5.4.99.5; HMM-score: 45.9)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01797; EC 5.4.99.5; HMM-score: 36.2)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01799; EC 5.4.99.5; HMM-score: 36.2)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01808; EC 5.4.99.5; HMM-score: 35.4)
  pseudaminic acid synthase (TIGR03586; EC 2.5.1.97; HMM-score: 28.5)
  Metabolism Amino acid biosynthesis Aromatic amino acid family putative chorismate mutase (TIGR01806; EC 5.4.99.5; HMM-score: 28.3)
  N-acetylneuraminate synthase (TIGR03569; EC 2.5.1.56; HMM-score: 18.3)
• TheSEED  :
  • 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
  • Chorismate mutase I (EC 5.4.99.5)
  Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
  and 3 more
  Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  Chorismate mutase I (EC 5.4.99.5)
  Amino Acids and Derivatives Aromatic amino acids and derivatives Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)  2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
  Amino Acids and Derivatives Aromatic amino acids and derivatives Phenylalanine and Tyrosine Branches from Chorismate  Chorismate mutase I (EC 5.4.99.5)
• PFAM:
  TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 287.3)
  and 3 more
  no clan defined CM_2; Chorismate mutase type II (PF01817; HMM-score: 74.1)
  TIM_barrel (CL0036) NeuB; NeuB family (PF03102; HMM-score: 32.7)
  no clan defined SPOB_ab; Sporulation initiation phospho-transferase B, C-terminal (PF14682; HMM-score: 15.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005725
  • TAT(Tat/SPI): 0.000718
  • LIPO(Sec/SPII): 0.000643
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161271 NCBI
• RefSeq: YP_494377 NCBI
• UniProt: A0A0H2XI85 UniProt

⊟Protein sequence

• MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRIYDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPEDTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSPYDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELLKEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDISAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDAGQQMDLDEFQAFYDELKPLADLYNAKKLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications