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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01852
  • pan locus tag?: SAUPAN004402000
  • symbol: SAOUHSC_01852
  • pan gene symbol?: aroA2
  • synonym:
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01852
  • symbol: SAOUHSC_01852
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 1757875..1758966
  • length: 1092
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGTAATAAATTAGAATCATACAGAAGTGAGATTGTATCACTGAATCATCAAATTTTA
    GACTTATTATCTAAACGTGGTGAACTAGCACAAAAAATTGGGGAAGAAAAATTAAAACAA
    GGTACACGTATTTATGATCCACAACGTGAAAAAGAAATGCTTAACGACTTAATCGATAGT
    AACAAAGGACCATTCAACGATAATACTATTAAGCAATTATTTAAAGAAATTTTCAAAGCC
    TCTACAGATTTACAAAAATCTGAAAATGAAAAACATTTATATGTATCACGTAAGTTGAAA
    CCTGAAGATACGATTGTAACATTTGATAATGGGGGCATTATAGGAGACGGCAATAAATCA
    TTTGTATTTGGGCCATGTTCAGTTGAATCATTTGAACAAGTTGAAGCTGTTGCTAAAAAC
    TTACATGCTAAAGGTGAAAAATTTATTCGTGGCGGTGCATTTAAACCACGTACATCACCA
    TATGATTTCCAAGGCCTAGGTGTTGAAGGACTTAAAATACTTAAACAGATTAAAGATAAA
    TATGATTTAAATGTTGTCAGCGAAATCGTAAATCCAAATGATTTTGAAGTGGCTGATGAG
    TATTTAGACGTATTCCAAATTGGTGCACGTAATATGCAAAACTTCGAGTTATTAAAAGAA
    GCTGGCCGTACGAAAAAGCCTATTCTATTAAAACGTGGTTTATCTGCTACAATCGAAGAG
    TTTGTTTATGCAGCTGAATACATTGCTTCACAAGGTAATCAAAACATTATTTTATGTGAA
    CGTGGAATCCGAACTTATGAAAAGGCGACACGTAACACTTTAGATATTTCAGCAGTACCA
    ATTTTAAAACAAGGTACACACTTACCAGTCATGGTAGATGTTACGCATAGTACAGGTCGT
    AAAGATATCATGTTACCAACTGCGAAAGCAGCATTAGCAGTTGGTGCTGATGGAGTTATG
    GCTGAGGTGCATCCAGATCCATCTGTTGCACTTAGTGATGCGGGTCAACAAATGGATTTA
    GATGAATTCCAAGCATTTTATGATGAATTAAAGCCTTTAGCTGATTTATATAACGCTAAA
    AAGTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01852
  • symbol: SAOUHSC_01852
  • description: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • length: 363
  • theoretical pI: 6.02863
  • theoretical MW: 40618
  • GRAVY: -0.44022

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-deoxy-7-phosphoheptulonate synthase (TIGR01361; EC 2.5.1.54; HMM-score: 381.7)
    and 11 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 170.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-8-phosphooctulonate synthase (TIGR01362; EC 2.5.1.55; HMM-score: 121.6)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 64.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01791; EC 5.4.99.5; HMM-score: 53.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01805; EC 5.4.99.5; HMM-score: 45.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01797; EC 5.4.99.5; HMM-score: 36.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01799; EC 5.4.99.5; HMM-score: 36.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01808; EC 5.4.99.5; HMM-score: 35.4)
    pseudaminic acid synthase (TIGR03586; EC 2.5.1.97; HMM-score: 28.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family putative chorismate mutase (TIGR01806; EC 5.4.99.5; HMM-score: 28.3)
    N-acetylneuraminate synthase (TIGR03569; EC 2.5.1.56; HMM-score: 18.3)
  • TheSEED  :
    • 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
    • Chorismate mutase I (EC 5.4.99.5)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
    and 3 more
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  Chorismate mutase I (EC 5.4.99.5)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)  2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Phenylalanine and Tyrosine Branches from Chorismate  Chorismate mutase I (EC 5.4.99.5)
  • PFAM:
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 287.3)
    and 3 more
    no clan defined CM_2; Chorismate mutase type II (PF01817; HMM-score: 74.1)
    TIM_barrel (CL0036) NeuB; NeuB family (PF03102; HMM-score: 32.7)
    no clan defined SPOB_ab; Sporulation initiation phospho-transferase B, C-terminal (PF14682; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005725
    • TAT(Tat/SPI): 0.000718
    • LIPO(Sec/SPII): 0.000643
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRIYDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPEDTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSPYDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELLKEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDISAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDAGQQMDLDEFQAFYDELKPLADLYNAKKLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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