PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08855 [old locus tag: NWMN_1578 ]
• pan locus tag^?: SAUPAN004307000
• symbol: NWMN_RS08855
• pan gene symbol^?: dnaB
• synonym:
• product: helicase DnaB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08855 [old locus tag: NWMN_1578 ]
• symbol: NWMN_RS08855
• product: helicase DnaB
• replicon: chromosome
• strand: -
• coordinates: 1745825..1747225
• length: 1401
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1745825..1747225) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1578

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  1141
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  1321
  1381
  
  ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTTATGCAA
  CACTTTGATTTAAATACGAATCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATT
  GGAACGCAAGCAGTGGGTTTATATCATTTTATGACTCAATTCGTTAAAGAAAGTCATAAT
  GAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
  TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACAT
  GATGAACAAGAAACACAATTTGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTT
  TTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAGTCGAGCATCGTCGATTTCAT
  GAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACA
  AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGAT
  ATTCCGATTAATGAACCGTACCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGAA
  ATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAGACATTGTTACCAAAGATGCA
  AAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
  GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAA
  GCACGCTCGTATTACTTAATGGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCT
  CCTGCTACATCATCTTCGGCGGGCAAATCTTCAGAAGTGAATCCAAAGCCACAAAGTGAT
  GAATGGTTTGAGTTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
  TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATTGAACGTGAAAAAATG
  TCTTTTGGCGTCATTAATATTTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTT
  CCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGAAAAAAGGTATTAAGACAGCT
  AAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
  AATTACCAAAAGCGTGGTTCATACTATGGGCAGCGTAATCGTATTTCAAAAGAAAAAACA
  CCTAAATGGTTAGAAAATAGAGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGC
  GTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTCTAGATAAATTATCTAAAAAA
  TGGGAGGAGGACAGTCAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS08855 [old locus tag: NWMN_1578 ]
• symbol: NWMN_RS08855
• description: helicase DnaB
• length: 466
• theoretical pI: 6.12276
• theoretical MW: 54532.6
• GRAVY: -0.661159

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 20.3)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 20.3)
• TheSEED: see NWMN_1578
• PFAM:
  no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 39.4)
  and 4 more
  DUF4144; protein structure with unknown function (PF13642; HMM-score: 16.1)
  DUF6241; Family of unknown function (DUF6241) (PF19754; HMM-score: 13.1)
  PA_Ig-like; Anthrax protective antigen, immunoglobulin-like domain (PF20835; HMM-score: 13.1)
  TPR (CL0020) PPP5; PPP5 TPR repeat region (PF08321; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8049
  • Cytoplasmic Membrane Score: 0.1923
  • Cell wall & surface Score: 0.0022
  • Extracellular Score: 0.0006
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002061
  • TAT(Tat/SPI): 0.00024
  • LIPO(Sec/SPII): 0.000242
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446455630 NCBI
• RefSeq: WP_000533485 NCBI
• UniProt: see NWMN_1578

⊟Protein sequence

• MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications