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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS08510 [old locus tag: NWMN_1513 ]
- pan locus tag?: SAUPAN004208000
- symbol: NWMN_RS08510
- pan gene symbol?: —
- synonym:
- product: collagenase-like protease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS08510 [old locus tag: NWMN_1513 ]
- symbol: NWMN_RS08510
- product: collagenase-like protease
- replicon: chromosome
- strand: -
- coordinates: 1677851..1679119
- length: 1269
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1261ATGAAGACAATAGAAGAGATTAAATCAACTCCTAAAACAGTTATGAAGAAACCAGAATTA
TTAGCACCTGCTGGAAACTTAGAAAAGTTAAAAATAGCAGTACATTATGGCGCTGATGCC
GTATTTTTAGGTGGTCAAGAATATGGATTACGTTCAAATGCTGATAATTTCACGATGGAA
GAAATAGCTGAAGGTGTTGAATTTGCGAACCGTTACGGTGCCAAAATTTATGTTACGACA
AATATTATTGCACATGATGAGAATATTGAAGGTCTAGAATCATATTTGCGTAATTTGGAA
AAGACTGGTGCGACAGGTATCATTGTTGCAGATCCTTTAATTATCGAAACATGTAAAGAA
GTTGCGCCAAAACTTGAAATTCATTTATCTACTCAACAATCACTTTCTAATTACAAAGCT
GTAGAATATTGGAAAGAAGAAGGATTGGATCGTGTTGTATTAGCACGTGAGACCGGCGCG
ATGGAAATGCGTGAAATGAAGGAAAAAGTAGATATTGAAATCGAAGCATTTATTCATGGT
GCTATGTGTATCGCCTATTCAGGTAGATGTACATTAAGTAATCATATGACTGCAAGGGAT
TCCAACAGAGGCGGTTGCTGTCAAAGTTGCCGTTGGGATTATGAATTATTAGAAGTTGAT
GATAATGGTGAACTTGATGTTTTTTATAATCAAGGTGAAGTTACACCGTTTGCGATGAGT
CCTAAAGATTTAAAATTAATCGAATCAATTCCTCAAATGATGGATATTGGTGTGGACTCA
TTAAAAATTGAAGGACGTATGAAGTCAATTCATTATATTGCAACAGTTGTCTCAGTATAT
CGTAAAGTCATTGATGCGTATGCGGCAGATCCTGACAACTTTAAGATTAATCCGGAATGG
TTAATAGAGTTAGATAAATGTGCAAATAGAGACACTGCACCAGCATTCTTTGAGGGAACA
CCTGGTTATGAAGAACAGATGTTTGGTCAACAACAATCTAAAAAATCACCATTTGATTTT
TGTGGTTTGGTATTAGACTATAATGAAGATACAAAAATTGCGACTATTCAACAACGAAAT
AACTTTAAACCAGGTCAAGAAATAGAATTCTTTGGTCCAGAAATTGAAACATTTACACAA
GTAGTAGAAGCAATTTATGATGAAGAAGGTAACAGCTTAGACGCGGCGCGCCATCCATTA
CAAATCGTTCAAATTAAAGTGGATCGCCCGATATATCCAAACAACATGATGAGAAAGGAA
ATTGGCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS08510 [old locus tag: NWMN_1513 ]
- symbol: NWMN_RS08510
- description: collagenase-like protease
- length: 422
- theoretical pI: 4.49587
- theoretical MW: 47654.8
- GRAVY: -0.38128
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined Peptidase_U32; Peptidase family U32 (PF01136; HMM-score: 286.7)and 3 morePeptidase_U32_C; Peptidase family U32 C-terminal domain (PF16325; HMM-score: 88.2)TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 15.7)no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005131
- TAT(Tat/SPI): 0.000485
- LIPO(Sec/SPII): 0.000603
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKEIG
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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