PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08440 [old locus tag: NWMN_1500 ]
• pan locus tag^?: SAUPAN004184000
• symbol: NWMN_RS08440
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08440 [old locus tag: NWMN_1500 ]
• symbol: NWMN_RS08440
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1666151..1666678
• length: 528
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1666151..1666678) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1500

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  
  ATGGGTTTAGTTCGCAAGTTTTTTATGCCGAATTCATATGTTCAATCAATATTTCAAATT
  GATTTAGACAAGTTAGTGGACAAAGGCGTTAAAGGTATTATTACAGATTTAGATAATACG
  CTAGTAGGTTGGGATGTTAAAGAACCTACAGAACGTGTTAAAGCATGGTTTAAGGAAGCT
  AATGAAAAAGGAATCACTATTACAATCGTGTCTAATAATAATGAGTCTCGTGTTGCTAGT
  TTTAGTCAGCATTTAGACATCGATTTTATTTTTAAAGCGAGAAAGCCAATGGGGAAAGCG
  TTTGATAAAGCAATAACTAAGATGAATATCAGACCAGATCAAACTGTTGTTATAGGTGAC
  CAAATGCTTACTGATGTATTTGGTGGTAATCGTCGAGGTCTATATACAATTATGGTTGTT
  CCAGTTAAACGAACTGATGGCTTTATTACTAAGTTTAATAGATTAATTGAAAGACGATTA
  TTACGTCATTTCAGTAAAAAAGGTTATATCACATGGGAGGAAAATTGA
  60
  120
  180
  240
  300
  360
  420
  480
  528
  

⊟Protein

⊟General

• locus tag: NWMN_RS08440 [old locus tag: NWMN_1500 ]
• symbol: NWMN_RS08440
• description: hypothetical protein
• length: 175
• theoretical pI: 10.4084
• theoretical MW: 20219.4
• GRAVY: -0.305714

⊟Function

• TIGRFAM:
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 166.3)
  and 27 more
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 81.4)
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 40.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 34.5)
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 34.2)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 31.3)
  phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 29.8)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 29)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 26.1)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 23.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 23.4)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 22.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 22.2)
  HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 22)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 21.4)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 21.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 21)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 18.8)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 18.1)
  HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 15.9)
  FkbH domain (TIGR01686; HMM-score: 15.9)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 15)
  mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 14.8)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 14.5)
  haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 13.8)
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 13.5)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 12.1)
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 11.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 38.7)
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 35.2)
  Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 34)
  PGP_phosphatase; Mitochondrial PGP phosphatase (PF09419; HMM-score: 32.8)
  and 4 more
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 26.1)
  Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 16.7)
  Glyco_hydro_tim (CL0058) Cellulase; Cellulase (glycosyl hydrolase family 5) (PF00150; HMM-score: 13.5)
  HAD (CL0137) S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003078
  • TAT(Tat/SPI): 0.000147
  • LIPO(Sec/SPII): 0.000352
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446447047 NCBI
• RefSeq: WP_000524902 NCBI
• UniProt: see NWMN_1500

⊟Protein sequence

• MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGWDVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFDKAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRLLRHFSKKGYITWEEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications