From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07760 [old locus tag: NWMN_1378 ]
  • pan locus tag?: SAUPAN003929000
  • symbol: NWMN_RS07760
  • pan gene symbol?: ndk
  • synonym:
  • product: nucleoside-diphosphate kinase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07760 [old locus tag: NWMN_1378 ]
  • symbol: NWMN_RS07760
  • product: nucleoside-diphosphate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1545215..1545664
  • length: 450
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1545215..1545664) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1378

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    GTGGAACGTACATTTTTAATGATTAAACCAGATGCAGTACAAAGAAATCTAATTGGTGAA
    GTAATTTCAAGAATTGAAAGAAAAGGACTAAAACTTGTCGGTGGTAAATTAATGCAAGTA
    CCAATGGAACTTGCTGAAACACATTATGGTGAACACCAAGGTAAACCATTTTATAATGAT
    TTAATTTCATTTATTACATCAGCACCAGTGTTCGCAATGGTAGTTGAAGGTGAAGATGCA
    GTTAATGTATCTAGACATATTATTGGCAGCACCAATCCTTCAGAAGCTTCACCAGGATCA
    ATTAGAGGTGATTTAGGTTTAACTGTTGGTAGAAATATCATTCACGGTTCAGATTCATTA
    GAGTCTGCTGAACGTGAAATTAATCTATGGTTTAATGAAAATGAAATTACTAGCTATGCT
    TCACCACGTGATGCATGGTTATATGAATAA
    60
    120
    180
    240
    300
    360
    420
    450


⊟Protein[edit | edit source]

Protein Data Bank: 3Q83
Protein Data Bank: 3Q86
Protein Data Bank: 3Q89
Protein Data Bank: 3Q8U
Protein Data Bank: 3Q8V
Protein Data Bank: 3Q8Y

⊟General[edit | edit source]

  • locus tag: NWMN_RS07760 [old locus tag: NWMN_1378 ]
  • symbol: NWMN_RS07760
  • description: nucleoside-diphosphate kinase
  • length: 149
  • theoretical pI: 4.82307
  • theoretical MW: 16574.6
  • GRAVY: -0.248322

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.4.6?  ExPASy
    Nucleoside-diphosphate kinase ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
  • TIGRFAM:
  • TheSEED: see NWMN_1378
  • PFAM:
    no clan defined NDK; Nucleoside diphosphate kinase (PF00334; HMM-score: 196.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9754
    • Cytoplasmic Membrane Score: 0.0045
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0199
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007039
    • TAT(Tat/SPI): 0.002696
    • LIPO(Sec/SPII): 0.000723
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMELAETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIRGDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]