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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS07540 [old locus tag: NWMN_1338 ]
- pan locus tag?: SAUPAN003859000
- symbol: NWMN_RS07540
- pan gene symbol?: thyA
- synonym:
- product: thymidylate synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS07540 [old locus tag: NWMN_1338 ]
- symbol: NWMN_RS07540
- product: thymidylate synthase
- replicon: chromosome
- strand: -
- coordinates: 1470645..1471601
- length: 957
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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901ATGTTGAATTCATTTGATGCAGCATATCACAGTCTTTGTGAAGAAGTTTTAGAAATAGGA
AATACACGAAATGATCGCACAAATACAGGTACGATTTCGAAATTTGGTCATCAACTTCGC
TTTGACTTATCTAAAGGATTTCCACTATTAACGACAAAGAAAGTTTCTTTTAAATTAGTA
GCAACCGAATTATTATGGTTCATTAAAGGAGATACAAACATCCAATACTTATTAAAATAT
AATAATAATATATGGAACGAATGGGCTTTTGAAAATTATATCAAATCAGACGAGTATAAA
GGTCCAGATATGACAGATTTCGGGCATCGTGCATTGAGTGATCCTGAATTTAACGAACAA
TATAAAGAACAAATGAAACAATTTAAGCAACGTATTCTTGAAGATGATACATTTGCGAAG
CAATTCGGGGATTTAGGAAATGTTTATGGTAAACAATGGCGAGATTGGGTTGATAAAGAT
GGTAATCATTTTGATCAACTTAAAACAGTAATTGAACAAATTAAGCATAATCCAGATTCA
AGGCGACACATCGTATCTGCATGGAATCCAACAGAAATTGATACAATGGCACTTCCGCCT
TGTCATACCATGTTCCAGTTTTATGTCCAAGATGGTAAGTTAAGTTGCCAGTTATACCAA
CGTAGCGCAGATATCTTTTTAGGTGTGCCATTTAATATCGCAAGCTACGCTTTATTGACA
CACCTTATTGCCAAAGAATGTGGACTTGAAGTGGGTGAATTTGTGCATACATTTGGAGAT
GCACATATTTATTCAAATCATATTGATGCGATTCAAACACAATTAGCACGTGAAAGCTTC
AATCCTCCAACATTAAAAATTAACAGTGACAAGTCTATTTTCGACATAAATTATGAAGAT
TTGGAAATTGTTGACTATGAATCACATCCAGCAATAAAAGCTCCAATAGCAGTGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS07540 [old locus tag: NWMN_1338 ]
- symbol: NWMN_RS07540
- description: thymidylate synthase
- length: 318
- theoretical pI: 5.37393
- theoretical MW: 36839.2
- GRAVY: -0.488679
⊟Function[edit | edit source]
- reaction: EC 2.1.1.45? ExPASyThymidylate synthase 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase (TIGR03284; EC 2.1.1.45; HMM-score: 529.9)and 1 morePurines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase, methanogen type (TIGR03283; EC 2.1.1.-; HMM-score: 70.8)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined Thymidylat_synt; Thymidylate synthase (PF00303; HMM-score: 393.8)and 3 moreNif11; Nif11 domain (PF07862; HMM-score: 16.8)RRM (CL0221) Tap-RNA_bind; Tap, RNA-binding (PF09162; HMM-score: 13.7)no clan defined LIAS_N; N-terminal domain of lipoyl synthase of Radical_SAM family (PF16881; HMM-score: 12.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008376
- TAT(Tat/SPI): 0.001204
- LIPO(Sec/SPII): 0.002181
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_RS00925 acetyl-CoA acetyltransferase [1] (data from MRSA252) NWMN_RS00930 3-hydroxyacyl-CoA dehydrogenase [1] (data from MRSA252) NWMN_RS03640 LysR family transcriptional regulator [1] (data from MRSA252) NWMN_RS04915 NAD(+) kinase [1] (data from MRSA252) NWMN_RS05380 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) NWMN_RS08930 pyruvate kinase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)