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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS00925 [old locus tag: NWMN_0167 ]
- pan locus tag?: SAUPAN001092000
- symbol: NWMN_RS00925
- pan gene symbol?: fadA
- synonym:
- product: acetyl-CoA acetyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS00925 [old locus tag: NWMN_0167 ]
- symbol: NWMN_RS00925
- product: acetyl-CoA acetyltransferase
- replicon: chromosome
- strand: -
- coordinates: 214222..215406
- length: 1185
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1141ATGCAAGAAGCATACATTGTAGCTTATGGGCGTTCAGCCGCAGCGAAAGCAAAGCAAGGC
GCATTATTCCACGAAAGACCTGATGATGTCGCAGCCAAAGTATTACAAGGCGTATTGAAA
CGTATTGACGGAAAATTCAATAAGAATATGATTGAAGATGTCATTGTTGGTACGGCTTTT
CCAGAAGGATTACAAGGCCAAAACATTGCACGAACGATTGCATTGCGTGCGGGATTATCT
GACACGGTACCGGGTCAAACAGTGAATCGCTACTGCTCATCAGGATTACAAACCATCGCG
ATTGCAGCCAATCAAATTATGGCTGGTCAAGGAGATATACTTGTAGCTGGTGGCGTTGAA
TTGATGAGTGCCGTACCAATGGGTGGCAACGAGCCCACAAACAATCCAACCTTACAATAT
GATGATATAGGTGCGTCATATCCTATGGGTTTAACTGCTGAAAATGTAGCATCCCAATTT
GACGTATCACGCGAAGATCAAGATGCTTATGCTGTCAGAAGTCATCAACGTGCCTATGAC
GCACAACGTGATGGTCGGTTCAAAGATGAAATTATTCCAATACAAGTAAACTCAGTTGAA
TATACAAACGCAGGACCAAAAGTACACACAAATATCTTTGACCAAGATGAATTTATACGC
CCTGACACCACGATGGAGGCATTAGCCAAATTACGTACAGTATTTAAAGCTGACGGCACT
GTGACTGCAGGAACATCTGCCCCACTTTCTGATGGTGCAGGATTTGTAGTTTTAATGTCT
GGAGATAAAGTGAAAGAACTCGGCGTGACACCTATTGCACGATTCGTTGGTTTTAAGGCA
GTAGGCGTTGACCCGAAAATTATGGGTATTGGGCCTGCATATGCGATTCCTGAAGTATTG
TCACTCAGCAATCTATCTGTTGAAGACATTGATTTGATCGAATTGAACGAAGCATTTGCT
TCTCAAACGATTGCATCTATTAAAGAAGTAGGTCTAGATATATCACGTACGAATGTGAAT
GGTGGCGCTATTGCTTTAGGTCATCCATTAGGTGCTACAGGCGCAATGTTAACCGCGCGT
TTACTTAATGAAATGGGTAGACGTCCCGATAGCCGTTACGGCATGGTTACGATGTGTATT
GGTGTCGGCATGGGTGCAGCTGCTATATTTGAATATGTGCGTTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS00925 [old locus tag: NWMN_0167 ]
- symbol: NWMN_RS00925
- description: acetyl-CoA acetyltransferase
- length: 394
- theoretical pI: 4.8083
- theoretical MW: 41804.3
- GRAVY: -0.0139594
⊟Function[edit | edit source]
- TIGRFAM: Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 454.8)and 4 moreFatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 348.8)3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 343.6)Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 198.1)Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 32.5)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 241.1)and 3 moreThiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 150.6)ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 22.6)P-loop_NTPase (CL0023) Podovirus_Gp16; Podovirus DNA encapsidation protein (Gp16) (PF05894; HMM-score: 12)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helix: 1
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007526
- TAT(Tat/SPI): 0.000561
- LIPO(Sec/SPII): 0.001163
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDGKFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIAANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQFDVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEFIRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFVGFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDISRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEYVR
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
NWMN_RS12420 50S ribosomal protein L4 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see NWMN_0167
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)