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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06615 [old locus tag: NWMN_1173 ]
  • pan locus tag?: SAUPAN003564000
  • symbol: NWMN_RS06615
  • pan gene symbol?: proS
  • synonym:
  • product: proline--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06615 [old locus tag: NWMN_1173 ]
  • symbol: NWMN_RS06615
  • product: proline--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1287514..1289217
  • length: 1704
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1287514..1289217) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1173

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAGCAATCCAAAGTTTTTATACCAACGATGCGTGACGTGCCATCAGAAGCAGAAGCA
    CAAAGTCATCGTTTATTATTGAAATCGGGTTTGATAAAACAAAGTACAAGTGGGATTTAT
    AGTTATTTACCGCTAGCAACACGTGTGTTAAATAATATTACTGCAATTGTGCGACAAGAA
    ATGGAACGTATCGATTCTGTTGAAATTTTAATGCCAGCGTTACAACAAGCTGAATTATGG
    GAAGAATCAGGACGTTGGGGTGCATATGGCCCAGAATTAATGCGTTTACAAGATAGACAT
    GGAAGACAATTTGCATTAGGTCCAACACATGAAGAATTAGTTACATCAATAGTAAGAAAT
    GAATTGAAATCATACAAACAATTACCGATGACATTATTCCAAATTCAATCTAAATTCCGT
    GATGAAAAGAGACCACGTTTTGGTTTACTTCGTGGGCGTGAATTTATTATGAAAGATGCA
    TATTCATTCCATGCTGACGAGGCATCATTAGATCAAACGTATCAAGATATGTATCAAGCG
    TATAGCCGTATTTTTGAGAGAGTTGGCATTAACGCAAGACCAGTAGTTGCAGATTCAGGT
    GCTATAGGCGGTAGCCATACACATGAATTTATGGCATTAAGTGCTATCGGTGAGGATACA
    ATCGTTTACAGTAAAGAAAGTGATTATGCTGCTAACATCGAAAAAGCAGAAGTCGTTTAC
    GAACCAAATCATAAGCATACTACTGTGCAACCTTTAGAAAAAATTGAAACACCAAATGTT
    AAGACTGCGCAAGAATTGGCAGACTTCTTAGGTAGACCAGTAGATGAAATCGTTAAAACG
    ATGATTTTCAAAGTTGATGGCGAATATATTATGGTTTTAGTGCGTGGCCATCATGAAATT
    AATGACATTAAATTAAAATCTTATTTCGGCACAGATAATATTGAATTAGCAACACAAGAC
    GAAATTGTTAATTTAGTTGGTGCAAATCCTGGTTCACTAGGTCCTGTAATTGATAAAGAA
    ATCAAAATTTATGCAGATAATTTTGTGCAAGATTTAAATAATTTAGTTGTCGGTGCTAAC
    GAAGATGGTTATCACTTAATTAATGTAAATGTAGGTAGAGACTTCAACGTTGATGAATAT
    GGCGATTTCCGTTTTATTTTAGAAGGCGAAAAGTTAAGTGATGGTTCAGGCGTTGCACAT
    TTTGCTGAAGGTATTGAAGTTGGTCAAGTATTCAAATTGGGTACTAAGTATTCAGAATCA
    ATGAATGCTACATTCTTAGATAACCAAGGAAAAGCTCAATCTTTAATTATGGGTTGTTAC
    GGAATTGGAATTTCTAGAACGCTAAGTGCGATTGTTGAACAAAATCACGATGATAATGGA
    ATTGTTTGGCCTAAATCAGTTACTCCGTTTGATTTACATTTAATTTCTATTAATCCTAAG
    AAAGATGATCAACGAGAACTAGCAGATGCACTATATGCTGAATTTAATACTAAATTTGAT
    GTGTTGTACGATGATCGTCAGGAACGTGCAGGTGTTAAATTTAATGATGCCGATTTAATT
    GGTTTACCACTGCGAATTGTTGTTGGTAAACGTGCATCGGAAGGTATTGTAGAAGTTAAA
    GAACGTTTAACAGGTGATAGCGAAGAAGTTCACATTGATGACTTAATGACTGTCATTACA
    AATAAATATGATAACTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1704

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS06615 [old locus tag: NWMN_1173 ]
  • symbol: NWMN_RS06615
  • description: proline--tRNA ligase
  • length: 567
  • theoretical pI: 4.84812
  • theoretical MW: 63862.8
  • GRAVY: -0.350088

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.15?  ExPASy
    Proline--tRNA ligase ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 741.6)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 129.9)
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 79.2)
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 40.3)
    Genetic information processing Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 35.9)
    Genetic information processing Protein synthesis tRNA aminoacylation Cys-tRNA(Pro) deacylase (TIGR00011; EC 3.6.1.-; HMM-score: 35)
    Genetic information processing Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 24.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 183.4)
    and 4 more
    no clan defined tRNA_edit; Aminoacyl-tRNA editing domain (PF04073; HMM-score: 95.9)
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 61)
    no clan defined DUF1543; Domain of Unknown Function (DUF1543) (PF07566; HMM-score: 23.2)
    FlaA; Flagellar filament outer layer protein Flaa (PF04620; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013355
    • TAT(Tat/SPI): 0.002282
    • LIPO(Sec/SPII): 0.001072
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS08865aldehyde dehydrogenase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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