PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS02455 [old locus tag: NWMN_0435 ]
• pan locus tag^?: SAUPAN002185000
• symbol: NWMN_RS02455
• pan gene symbol^?: gltC
• synonym:
• product: LysR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS02455 [old locus tag: NWMN_0435 ]
• symbol: NWMN_RS02455
• product: LysR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 485932..486816
• length: 885
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (485932..486816) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0435

⊟Phenotype

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⊟DNA sequence

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  ATGGAAATTAAACAATTAAGATATTTTATCGAGGTCGCTAAAAGAGAACATATCTCCGAA
  ACAGCACTTGAACTAAACATTGCTCAATCAGCGATAAGCCGTCAAATTACATTATTAGAA
  CAAGAACTTAATGTGTCTTTATTTAAAAAGCAAGGACGCAATATTACGCTCACATCTGAA
  GGGAAATTATTATTTAATGAAGCTCTGCGTATTATTGAACATTTAGATAGCACGATTGAA
  CAATTTCAAAGCCATGGATTAACTAAAAATAAAAGTATCTACATTGGCTATGATGAGAGT
  GACGTATCCCACATGTTACTACCACTTATCCAAACGTTTCATTTACAAAATGATACACAT
  GTCATACCGAGCTTATTAGAACATGACACAATTATAAATAGCGTATTAAATGGGAATATA
  GACATTGGTTTCACGGAACTTACACCAGAAATACGTAAACACAAACAATTACATATGCTA
  CCTTTATTCGAAGAGCACTATCATTTATATGCGCCTTCAGACGATCCAATTACGATGGCG
  ACACACCCACCATTAATTCAGTTTGAGCATAGTCATATTTATTGCTTGGCACCATTTGCC
  GAAACTGTAAAAAAACAATTGCGCAAGATTACTAAATCAGATGTATACACAATCAGTAGC
  CAACCACTTGCGCAGTATCTCCTTCGTCAAAAAGAAGGTTACATCATTTCATCACAAAAT
  ATACATTTACCAGAATCTAAATCATGGATAGATATAAAATTAGATCATACTGAATTAAAA
  AGAACGATATGTGCTATTACAAAGGAGCCTTATACAAAGAGTGATATTGGCATATTATTA
  ACACTTATTCAGCAATTAATGACTAAAACGTCTACATTCCATTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS02455 [old locus tag: NWMN_0435 ]
• symbol: NWMN_RS02455
• description: LysR family transcriptional regulator
• length: 294
• theoretical pI: 6.76432
• theoretical MW: 33942.8
• GRAVY: -0.257823

⊟Function

• TIGRFAM:
  putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 66.5)
  Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.3)
  Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.3)
  and 5 more
  Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 50.6)
  Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 40.5)
  Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 64.2)
  and 4 more
  PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 33.7)
  no clan defined Ribosomal_S13_N; Ribosomal S13/S15 N-terminal domain (PF08069; HMM-score: 17.2)
  HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 16.3)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by GltC, TF important in Glutamate metabolism: see NWMN_0435

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002278
  • TAT(Tat/SPI): 0.000185
  • LIPO(Sec/SPII): 0.000318
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446326061 NCBI
• RefSeq: WP_000403916 NCBI
• UniProt: see NWMN_0435

⊟Protein sequence

• MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELNVSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDVSHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHMLPLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTISSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDIGILLTLIQQLMTKTSTFH

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, GltC see NWMN_0435

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications