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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0513 [new locus tag: SACOL_RS02590 ]
  • pan locus tag?: SAUPAN002185000
  • symbol: gltC
  • pan gene symbol?: gltC
  • synonym:
  • product: transcriptional regulatory protein GltC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0513 [new locus tag: SACOL_RS02590 ]
  • symbol: gltC
  • product: transcriptional regulatory protein GltC
  • replicon: chromosome
  • strand: -
  • coordinates: 512593..513477
  • length: 885
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAATTAAACAATTAAGATATTTTATCGAGGTCGCTAAAAGAGAACATATCTCCGAA
    ACAGCACTTGAACTAAACATTGCTCAATCAGCGATAAGCCGTCAAATTACATTATTAGAA
    CAAGAACTTAATGTGTCTTTATTTAAAAAGCAAGGACGCAATATTACGCTCACATCTGAA
    GGGAAATTATTATTTAATGAAGCTCTGCGTATTATTGAACATTTAGATAGCACGATTGAA
    CAATTTCAAAGCCATGGATTAACTAAAAATAAAAGTATCTACATTGGCTATGATGAGAGT
    GACGTATCCCACATGTTACTACCACTTATCCAAACGTTTCATTTACAAAATGATACACAT
    GTCATACCGAGCTTATTAGAACATGACACAATTATAAATAGCGTATTAAATGGGAATATA
    GACATTGGTTTCACGGAACTTACACCAGAAATACGTAAACACAAACAATTAAATATGCTA
    CCTTTATTCGAAGAGCACTATCATTTATATGCGCCTTCAGACGATCCAATTACGATGGCG
    ACACACCCACCATTAATTCAGTTTGAGCATAGTCATATTTATTGCTTGGCACCATTTGCC
    GAAACTGTAAAAAAACAATTGCGCAAGATTACTAAATCAGATGTATACACAATCAGTAGC
    CAACCACTTGCGCAGTATCTCCTTCGTCAAAAAGAAGGTTACATCATTTCATCACAAAAT
    ATACATTTACCAGAATCTAAATCATGGATAGATATAAAATTAGATCATACTGAATTAAAA
    AGAACGATATGTGCTATTACAAAGGAGCCTTATACAAAGAGTGATATTGGCATATTATTA
    ACACTTATTCAGCAATTAATGACTAAAACGTCTACATTCCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    885

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0513 [new locus tag: SACOL_RS02590 ]
  • symbol: GltC
  • description: transcriptional regulatory protein GltC
  • length: 294
  • theoretical pI: 6.7243
  • theoretical MW: 33919.8
  • GRAVY: -0.258844

⊟Function[edit | edit source]

  • TIGRFAM:
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 66.5)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.4)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.4)
    and 5 more
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 50.6)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 40.5)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
  • TheSEED  :
    • GltC, transcription activator of glutamate synthase operon
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory DNA-binding regulatory proteins, strays  GltC, transcription activator of glutamate synthase operon
  • PFAM:
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 64.2)
    and 4 more
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 32.2)
    no clan defined Ribosomal_S13_N; Ribosomal S13/S15 N-terminal domain (PF08069; HMM-score: 17.2)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 16.2)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: 2-oxoglutarate; Glutamate
  • genes regulated by GltC, TF important in Glutamate metabolismRegPrecise
    activation
    gltC
    gltB > gltD
    transcription units transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002278
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000318
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELNVSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDVSHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLNMLPLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTISSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDIGILLTLIQQLMTKTSTFH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY (repression) regulon, GltC (activation) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 
    GltC(TF)important in Glutamate metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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