PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1921 [new locus tag: NWMN_RS11085 ]
• pan locus tag^?: SAUPAN005125000
• symbol: NWMN_1921
• pan gene symbol^?: —
• synonym:
• product: phage cI-like repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1921 [new locus tag: NWMN_RS11085 ]
• symbol: NWMN_1921
• product: phage cI-like repressor
• replicon: chromosome
• strand: +
• coordinates: 2128167..2128880
• length: 714
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331185 NCBI
• RefSeq: YP_001332955 NCBI
• BioCyc:
• MicrobesOnline: 3707509 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCA
  AATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACTTTAAGCGAT
  TATTTAAATTTACGTTCCAACCCCTCTCACGGCGTTATACAAAGGATAGCTGATGTTTTC
  GAGGTTGGTAAAAGCGACATAGATACTACATACAAAGACGATAACGACATCACTTCCATA
  TACAACAAACTCACACCTCCCCGCCAAGAAAACGTACTTAACTATGCAAATGAACAATTG
  GAAGAACAGAATTCTAAAGGAGATAACGTTGTAGATATTAATTCATATAAACAGGAGAAA
  ACTCCAGTTAACGTCAATGGTTGCGTCTCTGCTGGTGTAGGAGAACGTTTACACGATGAA
  ACGCTATTTACTGAAATGGTTAAAGGACCTATCCCCACACACGATTTAGCGTTAAAAGTA
  AATGGTGATTCTATGGAACCTATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACT
  CACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAG
  AAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAT
  CTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_1921 [new locus tag: NWMN_RS11085 ]
• symbol: NWMN_1921
• description: phage cI-like repressor
• length: 237
• theoretical pI: 5.05491
• theoretical MW: 27017.4
• GRAVY: -0.528692

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 41.6)
  Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 41.6)
  and 9 more
  signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 26)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 24.4)
  Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 16.7)
  Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 16.7)
  ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 15.9)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 12.5)
• TheSEED: data available for N315, NCTC8325, USA300_FPR3757
• PFAM:
  Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 60)
  and 14 more
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 42.8)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 29.3)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 24.2)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 21.8)
  HNF-1_N; Hepatocyte nuclear factor 1 (HNF-1), N terminus (PF04814; HMM-score: 20.7)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 16.9)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 14.9)
  PELOTA (CL0101) TSNR_N; Thiostrepton-resistance methylase, N terminus (PF04705; HMM-score: 13.4)
  PDDEXK (CL0236) PDDEXK_10; PD-(D/E)XK nuclease superfamily (PF07788; HMM-score: 13.2)
  HTH (CL0123) CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.1)
  YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 13)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 12.2)
  MarR; MarR family (PF01047; HMM-score: 12.1)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004779
  • TAT(Tat/SPI): 0.000511
  • LIPO(Sec/SPII): 0.000535
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222133 NCBI
• RefSeq: YP_001332955 NCBI
• UniProt: A0A0H3KDZ7 UniProt

⊟Protein sequence

• MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNLRSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEEQNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKVNGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDYDDLHFYRNESVRLIGKVIL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1921 > NWMN_1922 > NWMN_1923

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications